Evaluation des Immunophilins FKBP51 als therapeutisches Ziel in der Therapie der Osteoarthrose

Sonnenbrodt, Ruben

dc.contributor.advisor	Miosge, Nicolai Prof. Dr.
dc.contributor.author	Sonnenbrodt, Ruben
dc.date.accessioned	2023-04-21T15:54:34Z
dc.date.available	2023-05-10T00:50:10Z
dc.date.issued	2023-04-21
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14638
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9850
dc.format.extent	XXX Seiten	de
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Evaluation des Immunophilins FKBP51 als therapeutisches Ziel in der Therapie der Osteoarthrose	de
dc.type	doctoralThesis	de
dc.title.translated	Evaluation of the immunophilin FKBP51 as therapeutic target in the therapy of osteoarthritis	de
dc.contributor.referee	Schilling, Arndt Prof. Dr
dc.date.examination	2023-05-02	de
dc.description.abstracteng	Osteoarthritis (OA) is a degenerative disease leading to a destruction of the articular cartilage and the surrounding tissues of the affected joints. In the late stages it cumulates in a complete loss of joint function. New regenerative therapy concepts involving stem cells are emerging, promising a regeneration of the articular cartilage, but regneration of the diseased cartilage tends to be a great challenge due to limited intrinsic repair capacity. Chondrogenic progenitor cells (CPC) found in the articular cartilage in the late stages of OA show a regenerative potential that might be stimulated by drug therapy. Purpose of this experimental study was to investigate the role of the immunophilin FK506-binding protein 51 (FKBP51) in CPCs in order to evaluate its role as a potential pharmacological target. FKBP51 is a protein with many different functions, and known as an modulator of the NF-κB pathway, which might play a role in the progression OA. To investigate the function of the protein in CPCs I used the CRISPR/Cas-9 method with the aim to create a FKBP51 knockout and RNA-silencing with small interfering-RNAs. The results detected FKBP51 in CPCs for the first time on the level of mRNA and protein. The knockdown and knockout experiments showed results with different possibilities of interpretation. The results provide the foundation of further investigations to understand the importance of FKBP51 in CPCs.	de
dc.contributor.coReferee	Meyer, Thomas Prof. Dr.
dc.subject.eng	FKBP51	de
dc.subject.eng	osteoarthritis	de
dc.subject.eng	chondrogenic progenitor cells	de
dc.subject.eng	CRISPR/Cas	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-14638-0
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Orthopädie (PPN619876204)	de
dc.description.embargoed	2023-05-10	de
dc.identifier.ppn	1843379805
dc.notes.confirmationsent	Confirmation sent 2023-04-21T19:45:01	de

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