Interactions Within and Between Cytoskeletal Filaments

Lorenz, Charlotta

dc.contributor.advisor	Köster, Sarah Prof. Dr.
dc.contributor.author	Lorenz, Charlotta
dc.date.accessioned	2023-05-26T15:04:37Z
dc.date.available	2023-06-02T00:50:13Z
dc.date.issued	2023-05-26
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14685
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9908
dc.format.extent	XXX Seiten	de
dc.language.iso	eng	de
dc.subject.ddc	530	de
dc.title	Interactions Within and Between Cytoskeletal Filaments	de
dc.type	doctoralThesis	de
dc.contributor.referee	Köster, Sarah Prof. Dr.
dc.date.examination	2021-10-06	de
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	Cellular mechanics are determined by the cytoskeleton which consists of three different kind of biopolymers – actin filaments, microtubules and intermediate filaments – which form an intricate network. Each filament type and the interactions between these different filaments contribute with their specific function to the survival and proliferation of the cell. Thus, understanding these filaments and interactions between them is vital to develop treatment against malfunction which can lead to cell disease or death. Here, we study and quantify the interaction within and between cytoskeletal filaments with optical tweezers and numerical simulations. We find that interactions within intermediate filaments determine the filament’s mechanical response. These interactions can be tuned by the expression of different types of intermediate filaments, change in surrounding ion concentrations and phosphorylation. Studying the interactions between different filament types, specifically, microtubules and vimentin intermediate filaments, shows that electrostatic and hydrophobic effects contribute to the interactions. Numerical simulations confirm that the direct interactions between these two filament types stabilize dynamic microtubules. Thus, by tuning the interactions within as well as between filaments, the cell owns a tool box to adjust filament mechanics and dynamics.	de
dc.contributor.coReferee	Klumpp, Stefan Prof. Dr.
dc.contributor.thirdReferee	Kruss, Sebastian Prof. Dr.
dc.contributor.thirdReferee	Betz, Timo Prof. Dr.
dc.contributor.thirdReferee	Adio, Sarah Dr.
dc.contributor.thirdReferee	Zippelius, Annette Prof. Dr.
dc.subject.eng	Cytoskeleton	de
dc.subject.eng	intermediate filaments	de
dc.subject.eng	Monte Carlo simulations	de
dc.subject.eng	optical tweezers	de
dc.subject.eng	protein interactions	de
dc.subject.eng	force spectroscopy	de
dc.subject.eng	keratin	de
dc.subject.eng	vimentin	de
dc.subject.eng	microtubules	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-14685-1
dc.affiliation.institute	Fakultät für Physik	de
dc.description.embargoed	2023-06-02	de
dc.identifier.ppn	1846814944
dc.notes.confirmationsent	Confirmation sent 2023-05-26T15:15:01	de

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Fakultät für Physik (inkl. GAUSS) [926]

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