| dc.contributor.advisor | Posnien, Nico Dr. | |
| dc.contributor.author | Wiegleb, Gordon | |
| dc.date.accessioned | 2023-11-01T15:39:22Z | |
| dc.date.available | 2023-11-03T00:50:09Z | |
| dc.date.issued | 2023-11-01 | |
| dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14943 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-10159 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-10159 | |
| dc.format.extent | 147 | de |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject.ddc | 570 | de |
| dc.title | Gene expression divergence during Drosophila head development on single nuclei resolution | de |
| dc.type | cumulativeThesis | de |
| dc.contributor.referee | Posnien, Nico Dr. | |
| dc.date.examination | 2022-11-04 | de |
| dc.description.abstracteng | In this work, I optimized single-nuclei and single-cell RNA sequencing protocols for working with small amounts of input tissue. Using one of these protocols, I generated 14 single-nuclei RNA expression datasets. These data cover up to five different timepoints of EADs during late larval development. These timepoints span meaningful biological events from cell growth and proliferation to differentiation in Drosophila compound eye development. The datasets include data for the closely related non-model species, D. mauritiana and D. simulans. I show, that temporal information of EAD development is preserved in these datasets. With this, I hope to contribute a valuable resource for further developmental and evolutionary studies of the EAD. Using this data, I characterized developmental changes in cell population sizes and gene expression patterns between the different species, which is a crucial time for the development of the compound eye. Beyond general patterns, I find divergence in cell population sizes between D. mauritiana and D. melanogaster or D. simulans, respectively. I aimed to identify core regulating TFs within the different cell types of the EAD. Furthermore, I subset the data to explore differences in gene expression between these species within core retinal cell types. By comparing the results of these approaches, I identified several genes which present themselves as promising candidates for explaining the evolutionary changes in the morphology of the eye. | de |
| dc.contributor.coReferee | Papantonis, Argyris Prof. Dr. | |
| dc.subject.eng | Single-cell RNA sequencing | de |
| dc.subject.eng | Drosophila | de |
| dc.subject.eng | Head | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-ediss-14943-0 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.description.embargoed | 2023-11-03 | de |
| dc.identifier.ppn | 1870497139 | |
| dc.notes.confirmationsent | Confirmation sent 2023-11-01T19:45:01 | de |