Investigation of OPA1 in Mitochondrial Dynamics and Ultrastructure

Stuut, Christiaan

dc.contributor.advisor	Jakobs, Stefan Prof. Dr.
dc.contributor.author	Stuut, Christiaan
dc.date.accessioned	2024-03-21T11:58:14Z
dc.date.available	2025-01-24T00:50:08Z
dc.date.issued	2024-03-21
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/15178
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-10396
dc.format.extent	141	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	570	de
dc.title	Investigation of OPA1 in Mitochondrial Dynamics and Ultrastructure	de
dc.type	doctoralThesis	de
dc.contributor.referee	Jakobs, Stefan Prof. Dr.
dc.date.examination	2024-01-26	de
dc.description.abstracteng	Mitochondria are highly dynamic organelles with a complex ultrastructure. The mitochondrial inner membrane (MIM) is folded into cristae in which oxidative phosphorylation, a process to generate energy for the cell, takes place. Cristae are connected to the flat part of the inner membrane space by crista junctions (CJs). The size and shape of cristae and their junctions is tightly regulated by MICOS, F1Fo ATP synthase, and the dynamin-like GTPase OPA1. The eight isoforms of OPA1 can be proteolytically cleaved by OMA1 and YME1L to create a large number of different OPA1 fragments, that are either membrane bound to the MIM or are soluble in the intermembrane space. The exact localisation of OPA1, as well as its interactions with MICOS proteins, remains unknown. In this work, I analysed the localisation of OPA1 using multiple super-resolution microscopy techniques. I found that OPA1, similarly to MIC60, displays a coordinated clustered distribution along mitochondria, possibly localising to CJs. In human cells deficient of OPA1, I analysed the effect of OPA1-deficiency on CJs using electron tomography. Cells deficient of OPA1 contained slit-like CJs, that were on average over 120 nm in length. By overexpressing MIC10 in these cells, I showed that OPA1 but not MIC10 influences the length of CJs.	de
dc.contributor.coReferee	Richter-Dennerlein, Ricarda Prof. Dr.
dc.subject.eng	super-resolution microscopy	de
dc.subject.eng	STED microscopy	de
dc.subject.eng	mitochondrial dynamics	de
dc.subject.eng	OPA1	de
dc.subject.eng	mitochondria	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-15178-0
dc.affiliation.institute	Biologische Fakultät für Biologie und Psychologie	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.description.embargoed	2025-01-24	de
dc.identifier.ppn	1884047211
dc.identifier.orcid	0009-0003-3277-0544	de
dc.notes.confirmationsent	Confirmation sent 2024-03-21T12:15:01	de

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GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1199]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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