Massenspektrometrische Proteomanalyse der gingivalen Sulkusflüssigkeit im Vergleich zwischen Juvenil und Adult

by Mira Spaeth
Doctoral thesis
Date of Examination:2024-08-07
Date of issue:2024-07-08
Advisor:Prof. Dr. Philipp Meyer-Marcotty
Referee:Prof. Dr. Philipp Meyer-Marcotty
Referee:Prof. Dr. Henning Urlaub
Persistent Address: http://dx.doi.org/10.53846/goediss-10595

 

 

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Abstract

English

In this study, the proteome of the sulcus fluid was compared between juvenile and adult patients with regard to orthodontic tooth movement. The results are expected to help to identify some of the causes of the variations in the course and success of orthodontic treatment in order to optimize it in the long term through monitoring or the addition of appropriate proteins. The gingival sulcus fluid is well suited for detecting the proteins involved in tooth movement - it can be taken quickly and microinvasively. In addition, it consists mainly of proteins specifically found in this space. The samples were taken from four groups: adult-male, adult-female, juvenile-male and juvenile-female. In preliminary tests, an optimized method was developed: SP3 beads were used upstream of the mass spectrometric method to analyze the samples; these represent a new procedure for the optimal preparation and digestion of the proteins in the samples. This method proved to be extremely successful in detecting even low molecular weight proteins, which are often lost with conventional methods. No protein was found that occurred exclusively in one of the four groups, but proteins were found in significantly different amounts. An increase in aspartate aminotransferase, lactate dehydrogenase, ATP synthase and ADP/ATP translocase was found in the samples from the juvenile groups. Juvenile test subjects can thus exhibit an increased synthesis and secretion capacity. In the two adult groups, on the other hand, an increased occurrence of proteins involved in inflammatory processes, fewer proteins involved in protein synthesis and secreted proteins were detected. This not only reduces secretion, but also the possibility of producing new proteins. The method developed proved to be very successful overall. Although the qualitative results are not yet suitable for fulfilling the desire for the possibility of parallel monitoring of orthodontic therapy, the proteins just mentioned here, which occurred significantly more frequently in juvenile subjects, can serve as an indicator for faster tooth movement before the start of orthodontic therapy. Further investigations and illustrations of exact correlations between the quantity of the significantly different proteins found in this study, the speed of tooth movement during therapy and the stability of the results after the end of therapy can then further optimize orthodontic treatment.
Keywords: gingival sulcus fluid; orthodontic tooth movement; proteomics; proteomic analysis; OTM; Single-pot, solid-phase-enhanced sample preparation; tooth movement
Schlagwörter: orthodontische Zahnbewegung; Zahnbewegung; Proteomanalyse; gingivale Sulkusflüssigkeit
 
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