| dc.contributor.advisor | Betz, Timo Prof. Dr. | |
| dc.contributor.author | Münker, Till Moritz | |
| dc.date.accessioned | 2024-07-09T17:16:29Z | |
| dc.date.available | 2024-07-16T00:50:51Z | |
| dc.date.issued | 2024-07-09 | |
| dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?ediss-11858/15357 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-10592 | |
| dc.format.extent | 167 | de |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject.ddc | 530 | de |
| dc.title | Investigation of active mechanical properties in the cytoplasm of living cells | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Betz, Timo Prof. Dr. | |
| dc.date.examination | 2024-06-17 | de |
| dc.subject.gok | Physik (PPN621336750) | de |
| dc.description.abstracteng | Over the past decades, the study of intracellular active mechanical properties has experienced
a growth in interest. This is mainly caused by recognizing that vital cellular
functions rely on the intricate interplay between the viscoelastic mechanical properties
of the cytoplasm and the active force generated by the consumption of metabolic energy.
However, quantifying these properties poses significant challenges due to the complexity
of the physical quantities and the elaborate and low-throughput experimental methods
required for their investigation. In this work, we propose two techniques to meet these
challenges. Firstly, we introduce a mechanical fingerprint that reduces the complexity
of intracellular active mechanical properties to a set of six parameters. Demonstrated
initially on HeLa cells, the fingerprint accurately captured changes in mechanics upon
disrupting cytoskeletal components, showcasing its capability to represent intracellular
dynamics concisely. The investigation was then expanded to 7 diverse cell types, where
their unique fingerprint could identify individual cell types.
Further correlational analysis led to the introduction of a three-dimensional phase space
comprised of resistance, activity, and fluidity. Here, the positions in phase space correlated
with expected cell functions, underlining the fingerprint’s capability of accelerating
the investigation of such relations. Secondly, we introduced the Mean Back Relaxation
(MBR) as a novel statistical tool to determine the breaking of detailed balance in confined
systems. This approach was first established in a well-controlled model system, mimicking
the intracellular space. It was then applied to living cells, where we could observe
surprising relations between the MBR and intracellular activity. Strikingly, by deploying
this relation, we determined the mechanical properties of MDCK cells by purely passive
observations. We thus present an alternative approach for the quantification of intracellular
active mechanical properties. This will drastically reduce experimental complexity
and increase the experimental throughput. | de |
| dc.contributor.coReferee | Krüger, Matthias Prof. Dr. | |
| dc.contributor.thirdReferee | Janshoff, Andreas Prof. Dr. | |
| dc.contributor.thirdReferee | Huisken, Jan Prof. Dr. | |
| dc.contributor.thirdReferee | Sollich, Peter Prof. Dr. | |
| dc.contributor.thirdReferee | Mettin, Robert Dr. | |
| dc.subject.eng | Cell mechanics | de |
| dc.subject.eng | Optical tweezers | de |
| dc.subject.eng | Biophysics | de |
| dc.subject.eng | MBR | de |
| dc.subject.eng | Mechanical fingerprint | de |
| dc.subject.eng | Rheology | de |
| dc.subject.eng | Statistical mechanics | de |
| dc.subject.eng | Non equilibrium | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-ediss-15357-5 | |
| dc.affiliation.institute | Fakultät für Physik | de |
| dc.description.embargoed | 2024-07-16 | de |
| dc.identifier.ppn | 1895100097 | |
| dc.notes.confirmationsent | Confirmation sent 2024-07-09T19:45:01 | de |