Generation of human innervated engineered skeletal muscle

by Nina Rubik
Doctoral thesis
Date of Examination:2024-10-17
Date of issue:2024-10-10
Advisor:Dr. Maria-Patapia Zafeiriou
Referee:Prof. Dr. Wolfram-Hubertus Zimmermann
Referee:Prof. Dr. Bernd Wollnik
Sponsor:Hertha-Sponer-College of MBExC Göttingen
Persistent Address: http://dx.doi.org/10.53846/goediss-10779

 

 

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Abstract

English

In this medical thesis we made use of human induced pluripotent stem cells and organoid technique to establish a protocol for the generation of spinal motor neuron organoids. Additionally, spinal motor neuron organoids were fused with engineered skeletal muscle to create a model of a neuromuscular junction. Motor neuron commitment of our established protocol was assessed by gene expression analysis by RT(q)PCR and by protein analysis by whole mount immunofluorescence. After the characterization of spinal motor neuron organoids, light-activatable neuronal organoids were generated from an optogenetic stem cell line and fused with skeletal muscle tissue, creating innervated engineered skeletal muscle. Functionality of innervated engineered skeletal muscle was assessed in force of contraction analysis.
Keywords: induced pluripotent stem cells; organoid technique; spinal motor neuron organoid; engineered skeletal muscle; optogenetics
Schlagwörter: induced pluripotent stem cells; organoid technique; spinal motor neuron organoid; engineered skeletal muscle; optogenetics