Osteogene und angiogene in vivo Differenzierung auf mit MSC und HUVEC prävaskularisierten Hydroxylapatitmatrizes

Abstract

Autologous transplantation of bone tissue is the gold standard for treating bone defects. Since the complications and limitations of autologous transplantation are diverse, the development of alternatives is of great relevance for regenerative medicine. In the present experiments, it was possible to colonize xenogeneic HA matrices with mesenchymal and endothelial progenitor cells and to investigate the influence on angio- and osteogenesis in comparison to non-colonized control carriers. The expression of angiogenic and osteogenic markers was analyzed in vitro. Pre-cultured matrices were incubated subcutaneously in the flank area of ​​RNU rats for 14 and 28 days. The subsequent processing of the matrices included, on the one hand, the observation of PKH-labeled cells on a confocal laser scanning microscope, and, on the other hand, embedding them in Technovit® 9100, preparing thin sections and histological processing using in situ hybridization, osteopontin immunohistochemistry and staining according to Smith-Karagianes. Vascular formation and bone regeneration were demonstrably better on the colonized carriers compared to the non-colonized controls. The pre-cultivation of cells on matrices before implantation is recommended for optimized osteogenic differentiation and better bone regeneration. Due to their ubiquitous and easy isolation, as well as their good differentiation ability, MSC are particularly well suited for colonization and pre-cultivation on matrix implants. For a positive influence on angiogenic and osteogenic differentiation in vivo, it can be discussed that the colonization of matrices with MSC alone is more useful than the complex colonization with cocultures of mesenchymal and endothelial progenitor cells.