Coordinated Transcriptional Response to Stress-Related Disorders in Multiple Brain Regions: A Spatial and snRNA-sequencing Study in Mice

Otten, Joy

dc.contributor.advisor	Klengel, Torsten Prof. Dr. Dr.
dc.contributor.author	Otten, Joy
dc.date.accessioned	2024-10-22T10:11:24Z
dc.date.available	2024-10-29T00:50:08Z
dc.date.issued	2024-10-22
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/15565
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-10792
dc.format.extent	144	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	570	de
dc.title	Coordinated Transcriptional Response to Stress-Related Disorders in Multiple Brain Regions: A Spatial and snRNA-sequencing Study in Mice	de
dc.type	doctoralThesis	de
dc.contributor.referee	Papantonis, Argyris Prof. Dr.
dc.date.examination	2023-11-23	de
dc.description.abstracteng	Recent work has demonstrated specific, genome-wide transcriptional changes related to fear and anxiety in rodent animal models and post-mortem human brain samples on the level of individual brain regions and even cell types. However, whether these changes are limited to a defined number of brain regions and how these changes interact on the level of transcriptional connectivity remains unknown. To address this issue, we performed spatial transcriptomics RNAseq (stRNAseq) analyses in a classical auditory threat conditioning model in C57BL/6J mice. Our results establish a molecular atlas of a relevant mouse brain section based on stRNAseq data that closely resembles histologically defined brain regions. We further provide evidence for a significant transcriptional response across all brain regions investigated, with a prominent transcriptional response in the medial and lateral habenula, and the choroid plexus. The differentially expressed genes and pathways contribute to a better understanding of the molecular correlates of threat conditioning. In addition, network analyses harnessing regional differences in gene expression between brain regions suggest changes in transcriptional connectivity between cortical and subcortical brain regions in the early phase of threat memory consolidation.	de
dc.contributor.coReferee	Posnien, Nico Dr.
dc.subject.eng	Spatial Transcriptomics	de
dc.subject.eng	Auditory threat conditioning	de
dc.subject.eng	mouse model	de
dc.subject.eng	network analyses	de
dc.subject.eng	brain circuits	de
dc.subject.eng	chronic social defeat stress	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-15565-0
dc.affiliation.institute	Biologische Fakultät für Biologie und Psychologie	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.description.embargoed	2024-10-29	de
dc.identifier.ppn	1907044175
dc.identifier.orcid	0000-0003-4913-8230	de
dc.notes.confirmationsent	Confirmation sent 2024-10-22T10:15:01	de

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