Die Rolle der CHD1 Depletion im Prostatakarzinom bei der epithelial zu mesenchymalen Transition und der ossären Metastasierung

Flemming, Katharina

dc.contributor.advisor	Uhlig, Annemarie PD Dr.
dc.contributor.author	Flemming, Katharina
dc.date.accessioned	2025-01-17T16:41:51Z
dc.date.available	2025-02-21T00:50:07Z
dc.date.issued	2025-01-17
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/15744
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-10965
dc.format.extent	95	de
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Die Rolle der CHD1 Depletion im Prostatakarzinom bei der epithelial zu mesenchymalen Transition und der ossären Metastasierung	de
dc.type	doctoralThesis	de
dc.title.translated	The role of CHD1 depletion in prostate cancer during epithelial to mesenchymal transition and osseous metastasis	de
dc.contributor.referee	Schilling, Arndt Prof. Dr
dc.date.examination	2025-01-24	de
dc.description.abstracteng	In the treatment of advanced prostate cancer with androgen deprivation, almost all patients develop castration resistance after some time. The tumor growth continues despite ongoing therapy. In these castration-resistant tumor cells an increased depletion of the chromatin modulator CHD1 was observed. A link between CHD1 and androgen resistance can therefore be assumed. The aim of this study was to investigate the possible influence of CHD1 depletion on prostate carcinoma cells and the influence of androgen deprivation on the CHD1-depleted cells. CHD1-depleted LNCaP cells were analyzed at the protein and RNA level using various methods. A dependence of mesenchymal markers on the CHD1 depletion and on androgen deprivation was found. CHD1-depleted cells migrate more frequently and typical EMT markers are upregulated. This can already be observed in standard medium and is further enhanced in androgen deprivation. As prostate carcinoma cells often metastasize to the bone, a possible change in the tumour cells to osteotropic cells was also investigated. No change in knockdown was found here, but a clear upregulation of osteotropic markers in the CHD1-depleted cells in androgen deprivation. The results indicate that CHD1 depletion in cells is responsible for their more aggressive growth and increased metastasis. Androgen deprivation further enhances these properties, and androgen deprivation stimulates osteomimicry in the cells. It remains to be investigated how far these changes go and whether the CHD1 depleted tumor cells may begin to metastasize under androgen deprivation, which would mean that androgen deprivation would be contraindicated in patients with CHD1 depletion.	de
dc.contributor.coReferee	Dressel, Ralf Prof. Dr.
dc.subject.eng	CRPC	de
dc.subject.eng	Osteomimicry	de
dc.subject.eng	castrate-resistant prostate cancers	de
dc.subject.eng	Prostate cancer	de
dc.subject.eng	CHD1	de
dc.subject.eng	metastasis	de
dc.subject.eng	Androgen deprivation	de
dc.subject.eng	EMT	de
dc.subject.eng	epithelial to mesenchymal transition	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-15744-4
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Urologie / Andrologie - Allgemein- und Gesamtdarstellungen (PPN619876131)	de
dc.description.embargoed	2025-02-21	de
dc.identifier.ppn	191509741X
dc.notes.confirmationsent	Confirmation sent 2025-01-17T19:45:02	de

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