Development of a Bone Adhesive Material based on Diazirinyl-Collagen Mimetic Peptides

by Maria Castillo Orenes
Doctoral thesis
Date of Examination:2024-12-13
Date of issue:2025-02-05
Advisor:Prof. Dr. Johannes C. L. Walker
Referee:Prof. Dr. Johannes C. L. Walker
Referee:Prof. Dr Arndt Schilling
Referee:Prof. Dr. Andreas Janshoff
Referee:Prof. Dr Nadja Simeth
Referee:Prof. Dr. Kai Tittmann
Referee:Dr. Holm Frauendorf
Persistent Address: http://dx.doi.org/10.53846/goediss-11061

 

 

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Abstract

English

The treatment of small and inaccessible bone fractures with metallic implants is challenging. One potential solution to this issue is the use of an adhesive to adhere the affected fragments. In the search for new biocompatible bone adhesives, collagen-based materials have been widely explored as collagen type I is the major organic component of the extracellular matrix in bone tissue. However, immunogenic responses complicate the use of collagen derived from animal tissues at the clinical level. Collagen Mimetic Peptides (CMPs) are currently being investigated as candidates for the synthesis of artificial extracellular matrix scaffolds due to their reduced toxicity and ability to hybridise with natural collagen. Additionally, the use of a non-toxic crosslinker that can be activated on demand and enhance bone adhesive strength has been proposed. Diazirines are small heterocyclic groups that can cleave under UV irradiation to form electrophilic carbenes, which are known to undergo rapid crosslinking reactions in biological systems. This thesis explores and proposes strategies for the full chemical synthesis of a bone adhesive material resulting from the combination of CMPs and diazirines.
Keywords: Collagen Mimetic Peptides; Diazirines; Solid Phase Peptide Synthesis; Hydrogels; Bone adhesives; Collagen; Photocrosslinking