MINFLUX nanoscopy deep in (living) brain tissue

by Thea Moosmayer
Doctoral thesis
Date of Examination:2024-10-29
Date of issue:2025-02-06
Advisor:Prof. Dr. Dr. Stefan W. Hell
Referee:Prof. Dr. Dr. Stefan W. Hell
Referee:Prof. Dr. Tim Salditt
Persistent Address: http://dx.doi.org/10.53846/goediss-11064

 

 

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Abstract

English

Optical fluorescence microscopy offers intrinsic advantages that enable imaging in tissue, such as non-invasiveness and specificity of the fluorescence signal. However, the resolution and thus the information content obtainable with far-field optical microscopy is constrained by diffraction of light. This diffraction limit was overcome by super-resolution fluorescence microscopy techniques. In particular, MINFLUX fluorescence nanoscopy has proven to be a reliable method to achieve 3D resolution on the nanometer scale due to its photon-efficient localization concept, being ideally independent of fluorophore properties such as emission wavelength or absorption cross-section. While MINFLUX fluorescence nanoscopy enables the investigation of the spatial arrangement and spacing of molecules with nanometer precision, it has so far been restricted to measurements close to the coverslip and in less complex samples. Still, imaging of protein distributions in the physiologically relevant context of tissue with resoluti
Keywords: Superresolution; Deep tissue imaging; Nanoscopy; Fluorescence microscopy; Synapse; AMPA receptor; PSD95
 
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