Transformation of waste gases into biocommodities by syngas fermentation

by Tim Böer
Doctoral thesis
Date of Examination:2024-09-05
Date of issue:2025-05-28
Advisor:Prof. Dr. Rolf Daniel
Referee:Prof. Dr. Rolf Daniel
Referee:PD Dr. Michael Hoppert
Referee:Prof. Dr. Stefanie Pöggeler
Referee:Prof. Dr. Kai Heimel
Referee:Prof. Dr. Jan de Vries
Referee:Prof. Dr. Gerhard Braus
Persistent Address: http://dx.doi.org/10.53846/goediss-11231

 

 

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Abstract

English

This study describes a total of 13 novel gas-fermenting strains from four different bacterial genera. Three strains represent a new species. These acetogenic and carboxydotrophic hydrogenogenic strains were successfully isolated from environmental sources comprising river sediment, horse feces, compost, sludge from a biogas plant, hot spring, covering soil of a charcoal burning pile and digested sludge from a digestion tower of a wastewater treatment plant. This was achieved with mesophilic and thermophilic enrichment using either H2 + CO2 or methylated compounds (methanol, methylated glycines or methoxylated monoaromates) as substrates. The enriched bacterial community was assessed using taxonomic analysis based on the 16S rRNA marker gene. Enriched strains of interest were obtained in pure culture by isolation strategies using solid media or dilution series. In total, six mesophilic and seven thermophilic strains were isolated and subsequently analyzed by physiological and genomic characterizations.
Keywords: Acetogen; Syngas fermentation; Moorella; Sporomusa; Terrisporobacter; Acetoanaerobium; Wood-Ljungdahl pathway; Carboxydotrophic hydrogenogen
 
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