In-vivo-Biofilmakkumulation auf Prothesenkunststoffen

by André Becker
Doctoral thesis
Date of Examination:2025-06-17
Date of issue:2025-06-12
Advisor:Prof. Dr. Ralf Bürgers
Referee:Prof. Dr. Ralf Bürgers
Referee:PD Dr. Franziska Irene Haupt
Persistent Address: http://dx.doi.org/10.53846/goediss-11312

 

 

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Abstract

English

INTRODUCTION: 
Denture base resins, such as heat- and cold-polymerized PMMA, as well as newer materials like CAD/CAM-PMMA and Polyamide-12, are widely used in removable dentures. Their long-term oral presence makes them susceptible to microbial biofilm formation, which can contribute to oral and systemic diseases. MATERIALS AND METHODS: Multi-material test specimens were fabricated using the four materials to be tested. The surfaces were polished and half of the specimen were artificially aged. Surface roughness was then measured. Two artificially aged and two non-aged specimens were mounted into an upper jaw splint, which was worn by 20 participants for 24 hours. After removal, biofilm accumulation on the specimens was analyzed using fluorescence staining and fluorescence microscopy. AIM: This in vivo study aimed to determine whether initial biofilm accumulation differs among various denture base materials and to evaluate the influence of variables such as age, gender, intraoral position, and artificial aging on biofilm accumulation. RESULTS: Artificial aging generally reduced surface roughness in all materials except for Polyamide-12, which became significantly rougher. Thus surface roughness had no significant impact on biofilm levels. Cold-polymerized PMMA was excluded from the final comparison, because of autofluorescence no measurement was possible. No significant differences in biofilm accumulation were observed among the remaining three materials. Neither gender nor artificial aging influenced biofilm formation. In contrast, patient age and intraoral positioning of the test specimens were significant factors: older individuals exhibited greater biofilm accumulation, and posterior locations showed significantly more biofilm than anterior ones. CONCLUSION: The results in this study suggest that the initial oral biofilm accumulation is not significantly affected by the type of denture base material used. Material selection should therefore be based on other clinical criteria, such as mechanical properties, biocompatibility, and ease of processing. Additionally, the study indicates that the development of new materials like CAD/CAM-PMMA or Polyamide-12 does not currently offer clinical advantages in terms of biofilm resistance.
Keywords: denture base material; denture base resin; initial biofilm; biofilm accumulation; fluorescence microscopy; surface roughness
Schlagwörter: Biofilmakkumulation; Prothesenkunststoff
 
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