Zerebrale Kartierung der neuronalen Redox-Veränderung in einem Maus-Modell für das Rett-Syndrom

Cerebral mapping of neuronal redox changes in a mouse model of Rett syndrome

by Hendrik Woeste
Doctoral thesis
Date of Examination:2025-07-08
Date of issue:2025-06-24
Advisor:Prof. Dr. Michael Müller
Referee:Prof. Dr. Michael Müller
Referee:Dr. Ivan Prof Bogeski
Referee:Prof. Dr. Philipp Kaufmann
Persistent Address: http://dx.doi.org/10.53846/goediss-11348

 

 

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Abstract

English

As part of this study, changes in the neuronal redox balance in the brains of Rett mice are mapped and correlated with the progression of Rett syndrome. Fluorescence microscopy techniques (primarily ratiometric CCD camera imaging and two-photon microscopy) were used along with the optical redox indicator roGFP1. This redox indicator is already stably expressed in the Rett mice, allowing for optical analyses to be performend directly on acutely isolated brain tissue without the need for prior transfection or transduction. With this method it was possible to document the changes in the neuronal redox balance in different brain areas, in different ages and genotypes of mice.
Keywords: Rett syndrome; roGFP; fluorescence microscopy