Untersuchung der Phagozytosefähigkeit humaner Vollblutleukozyten in Abhängigkeit von der Stimulation durch Gummi arabicum

by Christin Freibrodt
Doctoral thesis
Date of Examination:2025-10-30
Date of issue:2025-10-24
Advisor:Prof. Dr. Roland Nau
Referee:Prof. Dr. Roland Nau
Referee:Prof. Dr. Carsten Lüder
Persistent Address: http://dx.doi.org/10.53846/goediss-11533

 

 

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Abstract

English

Background: Gum arabic, a polysaccharide exudate from Acacia senegal (L.)Willdenow trees, has already been used by African native people in natural medicine. Methods: Using whole-blood samples from young (20–35 years) and older (>80 years) healthy volunteers (each group n = 10), the effect of an aqueous solution of GA on phagocytosis of Escherichia coli was examined with a gentamicin protection assay. Whole-blood samples of each volunteer were stimulated with GA and as a control with CpG oligodeoxynucleotides (Toll-like receptor -9 agonists) for 2 h, then co-incubated with E. coli for 30 min and thereafter treated with gentamicin for up to 240 min to kill extracellular bacteria. Then, whole-blood cells were lysed with distilled water, and colony-forming units were counted by quantitative plating. Cytokine enzyme-linked immunosorbent assay for the detection of TNF-α and IL-6 was performed using the blood supernatant. Results: The GA concentration tested (20 mg/mL) did not affect the viability of eukaryotic cells. Phagocytosis of E. coli by whole-blood leukocytes derived from young (p = 0.008) and older (p = 0.004) healthy volunteers was increased by 120.8% (young) and 39.2% (old) after stimulation with GA. In contrast, CpG only stimulated the bacterial phagocytosis by cells derived from young volunteers (p = 0.004). Stimulation of whole blood with GA increased the intracellular killing of E. coli in young (p = 0.045) and older volunteers (p = 0.008) and induced a TNF-α release in whole blood collected from older volunteers but not from younger ones (p = 0.008). Conclusions: These data encourage the isolation of active compounds of GA and the initiation of clinical trials addressing the preventive effect of GA on bacterial infections.
Keywords: Acacia senegal (L.) Willdenow tree; gum arabic; phagocytosis; bactericidal effect; TNF-α; IL-6
Schlagwörter: Phagozyose; Gummi arabicum; TNF-α; IL-6; bakterizid; Leukozyten
 
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