Untersuchung der Phagozytosefähigkeit humaner Vollblutleukozyten in Abhängigkeit von der Stimulation durch Gummi arabicum

Freibrodt, Christin

dc.contributor.advisor	Nau, Roland Prof. Dr.
dc.contributor.author	Freibrodt, Christin
dc.date.accessioned	2025-10-24T15:18:11Z
dc.date.available	2025-11-27T00:50:06Z
dc.date.issued	2025-10-24
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/16299
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-11533
dc.format.extent	77	de
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Untersuchung der Phagozytosefähigkeit humaner Vollblutleukozyten in Abhängigkeit von der Stimulation durch Gummi arabicum	de
dc.type	doctoralThesis	de
dc.contributor.referee	Nau, Roland Prof. Dr.
dc.date.examination	2025-10-30	de
dc.description.abstracteng	Background: Gum arabic, a polysaccharide exudate from Acacia senegal (L.)Willdenow trees, has already been used by African native people in natural medicine. Methods: Using whole-blood samples from young (20–35 years) and older (>80 years) healthy volunteers (each group n = 10), the effect of an aqueous solution of GA on phagocytosis of Escherichia coli was examined with a gentamicin protection assay. Whole-blood samples of each volunteer were stimulated with GA and as a control with CpG oligodeoxynucleotides (Toll-like receptor -9 agonists) for 2 h, then co-incubated with E. coli for 30 min and thereafter treated with gentamicin for up to 240 min to kill extracellular bacteria. Then, whole-blood cells were lysed with distilled water, and colony-forming units were counted by quantitative plating. Cytokine enzyme-linked immunosorbent assay for the detection of TNF-α and IL-6 was performed using the blood supernatant. Results: The GA concentration tested (20 mg/mL) did not affect the viability of eukaryotic cells. Phagocytosis of E. coli by whole-blood leukocytes derived from young (p = 0.008) and older (p = 0.004) healthy volunteers was increased by 120.8% (young) and 39.2% (old) after stimulation with GA. In contrast, CpG only stimulated the bacterial phagocytosis by cells derived from young volunteers (p = 0.004). Stimulation of whole blood with GA increased the intracellular killing of E. coli in young (p = 0.045) and older volunteers (p = 0.008) and induced a TNF-α release in whole blood collected from older volunteers but not from younger ones (p = 0.008). Conclusions: These data encourage the isolation of active compounds of GA and the initiation of clinical trials addressing the preventive effect of GA on bacterial infections.	de
dc.contributor.coReferee	Lüder, Carsten Prof. Dr.
dc.subject.ger	Phagozyose	de
dc.subject.ger	Gummi arabicum	de
dc.subject.ger	TNF-α	de
dc.subject.ger	IL-6	de
dc.subject.ger	bakterizid	de
dc.subject.ger	Leukozyten	de
dc.subject.eng	Acacia senegal (L.) Willdenow tree	de
dc.subject.eng	gum arabic	de
dc.subject.eng	phagocytosis	de
dc.subject.eng	bactericidal effect	de
dc.subject.eng	TNF-α	de
dc.subject.eng	IL-6	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-16299-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.description.embargoed	2025-11-27	de
dc.identifier.ppn	1939450829
dc.notes.confirmationsent	Confirmation sent 2025-10-24T19:45:01	de

Files in this item

Name:Freibrodt_Stimulation Phagozytose.pdf

Size:1.114Mb

Format:PDF

View/Open

Name:: Freibrodt_Stimulation Phagozyt ...
Size:: 1.114Mb
Format:: PDF

View/Open

This item appears in the following Collection(s)

Human- und Zahnmedizin [3404]

Show simple item record