Advanced Correlation Spectroscopy and Nanofluidics in Single-Molecule Biophysics

Sakhapov, Damir

dc.contributor.advisor	Enderlein, Jörg Prof. Dr.
dc.contributor.author	Sakhapov, Damir
dc.date.accessioned	2025-10-28T08:44:48Z
dc.date.available	2025-11-04T00:50:08Z
dc.date.issued	2025-10-28
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/16308
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-11453
dc.format.extent	145	de
dc.language.iso	eng	de
dc.subject.ddc	571.4	de
dc.title	Advanced Correlation Spectroscopy and Nanofluidics in Single-Molecule Biophysics	de
dc.type	doctoralThesis	de
dc.contributor.referee	Grubmüller, Helmut Prof. Dr.
dc.date.examination	2025-03-28	de
dc.description.abstracteng	Fluorescence Correlation Spectroscopy (FCS) is a powerful and widelyused technique in biophysics for studying biomolecules and their interactionsat the single-molecule level. However, traditional FCS hasnotable limitations. First, it is highly sensitive to imperfections in theoptical system, which are often unavoidable in practical applications.In this work, we extend the use and uncover new applications of advancedFCS techniques—including antibunching FCS, PhotoinducedElectron Transfer (PET) FCS, Dual-Color FCS (2fFCS), and RotationalDiffusion FCS—to investigate a range of phenomena relevant to the lifesciences. These include the photophysics of fluorescent molecules, theintramolecular dynamics of Intrinsically Disordered Proteins (IDPs),and the docking-undocking dynamics of ribosomes with translocons.Secondly, while FCS techniques provide valuable insights, they arefundamentally ensemble-averaging approaches. True single-moleculetechniques, such as the Anti-Brownian Electrokinetic (ABEL) trap andsingle-molecule burst analysis, have emerged over the past decadesbut remain limited in widespread use due to the complexity of therequired instrumentation. Recent advancements in nanofluidics, fueledby knowledge transfer from the field of microfabrication to the lifesciences, offer promising solutions. To harness this potential, we havedeveloped a nanofluidic-based fabrication process from the groundup, aiming to simplify instrumentation and make single-molecule biophysicsmore accessible. Our ultimate objectives include the trappingof single biomolecules within nanochannels and measuring electriccharges of bimolecules under physiological conditions.	de
dc.contributor.coReferee	Rodnina, Marina Prof. Dr.
dc.subject.eng	Biophysics	de
dc.subject.eng	Nanofluidics	de
dc.subject.eng	FCS	de
dc.subject.eng	Nanofabrication	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-16308-8
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.description.embargoed	2025-11-04	de
dc.identifier.ppn	1939631882
dc.notes.confirmationsent	Confirmation sent 2025-10-28T08:45:02	de

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GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1269]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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