Role of bacterial rhamnolipids in plant tolerance to abiotic stresses

Bouchrati, Maoulida Ali

dc.contributor.advisor	Feussner, Ivo Prof. Dr.
dc.contributor.author	Bouchrati, Maoulida Ali
dc.date.accessioned	2025-10-29T09:40:04Z
dc.date.available	2025-11-07T00:50:09Z
dc.date.issued	2025-10-29
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/16312
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-11352
dc.format.extent	283	de
dc.language.iso	eng	de
dc.subject.ddc	570	de
dc.title	Role of bacterial rhamnolipids in plant tolerance to abiotic stresses	de
dc.type	doctoralThesis	de
dc.contributor.referee	Ischebeck, Till Prof. Dr.
dc.date.examination	2024-11-05	de
dc.description.abstracteng	Rhamnolipids (RLs), glycolipids produced by Pseudomonas and Burkholderia species, have been described as potential biocontrol agents due to their antagonistic activity against pathogens and their ability to induce plant defence responses. To date, however, certain aspects of RLs remain to be elucidated, notably their effects under abiotic stress and their mechanism of perception by the plasma membrane (PM). Previous results have suggested that the perception of RLs involves an interaction with PM lipids. In this thesis, we showed that RLs do not protect Arabidopsis against heat stress. Lipidomic analyses revealed that the application of RLs do not induce any major change in the composition of in vivo PM lipids. In addition, we showed that the effectiveness of RLs in inducing resistance to Pseudomonas syringae is compromised in the longevity assurance gene (lag) one homologous gene 2 (loh2) mutant, a ceramide synthetase knockout mutant. Lipidomic analysis showed that loh2 had an altered SL composition, but that its phospholipid and sterol composition was similar to that of Wt. Proteomic analysis revealed that the loss of LOH2 had no major impact on the composition of the Arabidopsis PM proteome. Taken together, these data indicate that the reduction in RL efficiency in the loh2 mutant is not due to changes in PM lipid composition, but rather to a potential influence of membrane lipids on signalling processes.	de
dc.contributor.coReferee	Fauconnier, Marie-Laure Prof. Dr.
dc.subject.eng	Rhamnolipids	de
dc.subject.eng	Lipidomic	de
dc.subject.eng	Arabidopsis thaliana	de
dc.subject.eng	Plasma membrane	de
dc.subject.eng	Plant immunity	de
dc.subject.eng	Photosynthesis	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-16312-0
dc.affiliation.institute	Biologische Fakultät für Biologie und Psychologie	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.description.embargoed	2025-11-05	de
dc.identifier.ppn	1939712505
dc.notes.confirmationsent	Confirmation sent 2025-10-29T09:45:01	de