Browsing Zentren & Graduiertenschulen by Advisor & Referee "Rehling, Peter Prof. Dr."
Now showing items 21-40 of 48
-
Analysis of mitochondrial transcription and replication on the single nucleoid level
(2019-03-13)Human mitochondrial DNA (mtDNA) is a circular molecule encoding for 13 subunits of the respiratory chain that are essential for its maintenance. Inside the mitochondria, mtDNA is associated with various proteins, forming ... -
Development of Nanobodies to Image Synaptic Proteins in Super-Resolution Microscopy
(2018-10-17)In the recent decades, super resolution microscopy substantially contributed to the investigation of subcellular structures. A prominent example is the molecular organization of the synapse which is known to be vital for ... -
Interactions and functions of RNA-binding proteins
(2018-04-13)Alongside several well-known modifications in DNA and proteins, more than 100 different types of chemical modification are also found in cellular RNAs. RNA modifications can influence the secondary structure and interactions ... -
Molecular Characterization of the Mitochondrial Presequence Translocase
(2018-01-31)In this thesis, multiple pore-lining amino acid residues of the mitochondrial presequence translocation channel Tim23 were identified as constituents of the channel’s cation filter by combining single channel electrophysiology ... -
Import of proteins along the presequence pathway
(2017-12-01)The aim of this study was to investigate the mechanism of protein transport by the presequence translocase (TIM23) in the inner mitochondrial membrane. Presequencecontaining proteins are imported through the translocase ... -
Characterization of RNA-modifying enzymes and their roles in diseases
(2017-11-29)More than 150 types of chemical modifications have been identified in cellular RNAs (collectively called ‘’the epitranscriptome’’), and such modifications have emerged as important regulators of gene expression. Despite ... -
Structural and biophysical characterization of human pyruvate dehydrogenase multi-enzyme complex
(2017-11-24)Pyruvate dehydrogenase multi-enzyme complex (PDHc) is an assembly of multiple copies of four different proteins. Together they carry out the oxidative decarboxylation of pyruvate and generate acetyl-CoA and NADH, which are ... -
Function of the INA complex in assembly of the mitochondrial oxidative phosphorylation system
(2017-07-11)The majority of cellular ATP is produced by the mitochondrial F1Fo-ATP synthase, also called complex V, which consists of nuclear- and mitochondrial-encoded subunits. In order to form a functional enzyme, its proteins ... -
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
(2016-12-13)Ectopic overexpression of fluorescent fusion proteins for live cell imaging studies often leads to a multitude of artefacts, but protein expression at endogenous levels in mammalian cells was difficult to achieve so far. ... -
Structural and functional investigation of cargo recognition by exportins
(2016-11-02)Nucleocytoplasmic exchange is essential for eukaryotic cells. It proceeds through nuclear pore complexes and is largely mediated by Importin β-like nuclear transport receptors (Impβ-like NTRs). According to the direction ... -
Mitochondrial protein assemblies: Biogenesis of the cytochrome c oxidase and mitophagic signaling complexes
(2016-09-05)Mitochondrial physiology requires a constant balance between biosynthesis and degradation events. This thesis addresses both mitochondrial biogenesis, exemplified by cytochrome c oxidase assembly, and mitochondrial removal ... -
Dynamic changes in cytochrome c oxidase assembly and organization
(2016-08-24)The respiratory chain in the inner membrane of the yeast mitochondrion is organized as a network of individual complexes and large supercomplex structures. These supercomplexes are composed of dimeric complex III and one ... -
Investigation of prokaryotic immune defense system with quantitative and structural mass spectrometry
(2016-02-26)Bacteria and archaea have evolved an adaptive and heritable immune defense system comprising a CRISPR (clustered regularly interspaced short palindromic repeats) locus and Cas (CRISPR-associated) proteins that targets ... -
Analysis of Mdm38 function in mitochondrial translation
(2015-11-05)The data presented in this thesis demonstrate that binding of Mdm38 to ribosomes has conserved characteristics in yeast and E. coli. To gain further insights into potential binding partners, an in vivo photo cross-linking ... -
Nuclear export of actin: A biochemical and structural perspective
(2015-08-18)Macromolecular exchange between the nucleus and the cytoplasm is mainly mediated by the RanGTP-dependent nuclear transport receptors (NTRs) of the Impβ family. NTRs are classified as importins and exportins according to ... -
Rab26 mediates selective targeting of synaptic vesicles to the autophagy pathway
(2015-02-26)In recent years it has been shown that macroautophagy regulates the turnover of postsynaptic receptors and modulates presynaptic neurotransmission. However it is still unclear whether the presynaptic protein turnover is ... -
Analysis of early steps in Assembly of Cytochrome c Oxidase
(2014-12-18)Cytochrome c oxidase (COX) is the terminal enzyme of the respiratory chain. Due to its multimeric nature, assembly and maturation requires the concerted action of over 20 assembly factors in yeast. COX is composed of both ... -
Residue level characterization of molecular interactions of intermembrane space domains governing the preprotein import into the mitochondrial matrix
(2014-12-10)Mitochondrial matrix targeting proteins are translated as preproteins (carrying an N-terminal 20-50 residue presequence) in the cytoplasm. Post-translationally, they are imported into the mitochondrial matrix through ... -
Biochemical characterization of the Nup62⋅58⋅54 nucleoporin complex and mutational analysis of the exportin CRM1
(2014-10-15)Nuclear pore complexes (NPCs) are giant molecular machines embedded in the double membrane that surrounds the eukaryotic nucleus. Deciphering the molecular structure of the NPCs is critical to our understanding of both ... -
Exploration of cargo spectrum and NES patterns recognized by the exportin CRM1
(2014-10-15)The nuclear envelope (NE) subdivides eukaryotic cells into a nuclear and a cytoplasmic compartment, forcing material exchange between these two compartments to proceed through the nuclear pore complexes (NPCs). While ...