Browse by type "doctoralThesis"
Now showing items 21-40 of 1233
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Disinhibitory circuit motifs in the mouse primary somatosensory (barrel) cortex
(2025-02-24)The barrel cortex is the part of the rodent neocortex where the tactile information from the whiskers is processed. In the barrel cortex, each whisker is represented by a single cortical column isomorphic to the whiskers ... -
Auditory nerve function in mouse models of ATP11A and CABP1-CABP2 related hearing loss
(2025-02-10)Hearing loss can be caused by varied factors affecting diverse structures of the auditory system such as the middle ear, the stria vascularis, the outer hair cells (OHC), inner hair cells (IHC), the spiral ganglion neurons ... -
Fluorescence Correlates of Single Vesicles to Analyze the Behavior of Membrane-manipulating Proteins
(2025-01-24)Soluble NSF-attachment protein receptors (SNAREs) are integral to eukaryotic membrane fusion, providing the necessary energy to surmount the repulsive forces between two opposing membranes. This is achieved through the ... -
The role of RNA in synaptic physiology and neurodegeneration in synucleinopathies
(2025-01-23)Synucleinopathies are neurodegenerative disorders characterised by the pathological accumulation and aggregation of the protein alpha-synuclein (aSyn). Parkinson’s disease (PD), Dementia with Lewy bodies (DLB), multiple ... -
From a First Seizure to Chronic Epilepsy – A Structural and Functional Study
(2025-01-23)Diagnosing epilepsy after a first unprovoked seizure is challenging, especially in patients without detectable lesions on standard magnetic resonance imaging (MRI) and without interictal epileptiform discharges (IEDs) ... -
Structure, Mechanism and Regulation of human UMP Synthase
(2025-01-10)Enzymes are of critical importance for a large range of biochemical reactions, catalyzing the conversion of a substrate to a product by lowering the energy of the transition state. The resulting rate acceleration in ... -
Rate-limiting steps of autophagy initiation
(2024-12-23)Autophagy research has progressed tremendously since the identification of ‘ATG’ genes and proteins responsible for maintaining the process. A dissection of general functions of all proteins involved was possible. However, ... -
Investigation of the dual role of mL62 during the mitoribosome assembly and rescue
(2024-12-19)Mitochondria are eukaryotic organelles, which have their own DNA (mtDNA) that encode for 13 proteins that are essential for the oxidative phosphorylation. The mitochondrial ribosome (mitoribosome) is responsible for the ... -
Characterization of VAPA at the inner nuclear membrane: localization, membrane insertion and nuclear interactome
(2024-12-19)Tail-anchored (TA) proteins are type IV single-pass membrane proteins characterized by a long hydrophobic transmembrane domain (TMD) positioned very close to a notably short C-terminus facing the lumen. Due to their topology, ... -
Transport mechanisms of the putative vesicular nucleotide transporter VNUT
(2024-12-13)The vesicular nucleotide transporter VNUT (SLC17A9) is thought to refill recycled synaptic vesicles and other secretory vesicles with adenosine triphosphate (ATP) for chemical signal transmission. ATP translocation by VNUT ... -
The dynamics of singularities in passive, driven, and active matter
(2024-12-13)Out-of-equilibrium statistical physics studies the time evolution of large collections of particles in terms of a reduced number of meso- and macroscopic observables. Their nature emerge from of the underlying microscopic ... -
Mechanisms of chromosome segregation in mammalian oocytes
(2024-12-10)Errors in chromosome segregation in human females often result in eggs with the wrong number of chromosomes, a condition known as aneuploidy. The rate of aneuploidy increases dramatically in women with age, particularly ... -
Advancements in Nanobody Applications: From Live Synaptic Imaging to Multiplexed Fluorescence Microscopy and Nanoscale Topography Mapping
(2024-12-09)Fluorescence microscopy facilitates biological research by enabling straightforward visualization of fluorescently labeled proteins in live and fixed samples. Despite significant progress in microscopy techniques, including ... -
PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Formation, regulation and dynamics of phase separated postsynaptic density condensates with Tau and Fyn
(2024-11-22)Alzheimer’s disease is characterized by memory loss and cognitive impairments. The cognitive deficits and synaptic dysfunction are associated with the excitotoxicity mediated by NMDA (N-methyl-D-aspartate) receptors on the ... -
Structural insight of MODAG-005 on 𝛼-Synuclein aggregates as a novel PET tracer
(2024-11-15)Neurodegenerative disorders, including Parkinson's disease (PD) and related synucleinopathies, are characterized by the accumulation of pathological protein aggregates in the brain. Among the key proteins implicated in ... -
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Interplay of proteins and lipids during endoplasmic reticulum-associated protein degradation
(2024-11-14)The endoplasmic reticulum controls lipid homeostasis due to its central role in lipid biosynthesis and its regulation. Aberrant lipid composition and membrane properties in the endoplasmic reticulum interfere with its ... -
Generation of innervated engineered human myocardium (iEHM) from human induced pluripotent stem cells to elucidate the mechanism underlying neuro-cardiac pathologies
(2024-11-12)Brain and heart consist of networks of excitable cells, the function of which is orchestrated by a complex interplay of ionic currents. Mutations in ion channels can predispose patients to cardiocerebral channelopathies ... -
Cell death signaling in the Arabidopsis chitin receptor mutant cerk1-4
(2024-11-07)Plant surface immunity is mediated by pattern recognition receptors (PRRs) that recognize con-served molecules derived from microorganisms. In Arabidopsis, CERK1 is the main component of the receptor complex that binds ...