Browsing Zentren & Graduiertenschulen by Advisor & Referee "Ficner, Ralf Prof. Dr."
Now showing items 1-20 of 73
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Molecular mechanisms and cellular functions of RNA methyltransferases targeting non-coding RNAs
(2024-12-23)Cellular RNAs contain diverse modified nucleotides that regulate their structure, stability, biogenesis and molecular functions. In abundant non-coding RNAs such as ribosomal (r)RNAs, transfer (t)RNAs and small nuclear ... -
Function and homeostasis of non-proteinogenic amino acids in Bacillus subtilis
(2024-07-23)The gram-positive soil bacterium Bacillus subtilis lives in a constantly changing environment, requiring rapid adaptation to survive and thrive. This forces the bacteria to adapt to nutrient availability or stressful ... -
UV and chemical crosslinking mass spectrometry for the analysis of protein-nucleic acid interactions
(2024-07-12)Protein-nucleic acid interactions are a key part of essential cellular processes and their disturbance often results in the development of diseases. Crosslinking mass spectrometry (XL-MS)-based approaches have become ... -
Regulation of human 48S initiation complex formation
(2024-04-23)During the initiation phase of protein synthesis in humans, the small ribosome subunit interacts with initiator tRNA (tRNAi) and at least ten different initiation factors (eIFs) to form the initiation complex. The initiation ... -
Structural and functional investigation of tRNA guanine transglycosylase
(2023-11-17)In this thesis, two novel structures were presented, jointly illuminating dimerization and tRNA binding by human TGT. A first structure, obtained by X-ray crystallography, allowed to give a thorough account of the atomic ... -
Validation of novel protein-protein interactions in Bacillus subtilis
(2023-01-12)Protein-protein interactions are the basis for many biological processes in the cell. Understanding these interactions is crucial for the general analysis of how the cell functions and the potential identification of ... -
Assembly pathway of the fungal COP9 signalosome
(2022-12-20)The conserved eight-subunit COP9 signalosome (CSN complex) controls the exchange of E3 ubiquitin cullin RING ligase receptors, and therefore the specificity of protein degradation in eukaryotic cells and it is required ... -
Functional and Structural Characterization of the DEAH-box Helicase Prp43
(2022-11-24)RNA helicases of the DEAH-box family fulfill essential functions in various aspects of RNA metabolism by reorganizing structured RNAs and RNPs through ATP-dependent motility in 3’ to 5’ direction along single-strands. Prp43 ... -
The role of GTPases in human mitochondrial ribosome biogenesis
(2022-05-03)Mitochondria are essential organelles of eukaryotic cells, which produce the vast majority of the cellular ATP via oxidative phosphorylation (OXPHOS). The mitochondrial gene expression machinery contributes to the formation ... -
Structural and functional characterisation of tRNA modifying enzymes
(2021-09-10)Posttranscriptional and posttranslational modifications are key regulatory mechanisms to expand biological properties of proteins and nucleic acids in living cells. A tremendous number of chemical modifications is found ... -
Characterization of Acyltransferases in Neutral Lipid Synthesis
(2021-04-23)Wax ester (WE) are neutral lipids, which consist of a fatty acid and a fatty alcohol moiety connected by an ester bond. WE are synthesized via two reactions from acyl-coenzyme A (CoA)/acyl carrier protein (ACP) substrates. ... -
Investigation of DNA in Cell Nuclei by Combined Imaging Techniques
(2021-03-31)Biological cells are the basic structural and functional units of all organisms. There are approximately 60 trillion eukaryotic cells within a human body alone. A common feature among eukaryotic cells is the nucleus, a ... -
Molecular insights into the roles of RNA helicases during large ribosomal subunit assembly
(2021-02-25)RNA helicases are enzymes present in all domains of life that are important in many aspects of RNA metabolism. They harbor a conserved helicase core that uses the energy of NTP binding and hydrolysis to change affinity to ... -
Signal transduction by the essential nucleotide second messenger cyclic di-AMP in Bacillus subtilis
(2021-01-15)Bacillus subtilis, as a soil inhabitant, has to adapt to rapidly changing osmotic conditions. The response to these osmotic changes represents one of the most critical issues in the lifecyle of bacteria, and the tight ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Structure Based Search for Novel Nuclear Export Inhibiting Anti-Tumor Drugs
(2020-07-28)The intracellular distribution of proteins and ribonucleoproteins is a fundamental process in all eukaryotes that is critically vital for the normal functionality of the eukaryotic cell. In the last decades, it has been ... -
Influence of Ions on the Assembly of Vimentin Intermediate Filaments
(2020-07-10)The human body consists of many different cell types, which all exhibit a cytoskeleton. This cytoskeleton is mainly composed of three different types of protein filaments, as well as cross-linkers and motor proteins. The ... -
Investigation of the effects of the splicing inhibitor Pladienolide B and cancer-related mutations in the SF3b1 protein on pre-mRNA splicing in vitro
(2020-06-11)Pre-mRNA splicing is catalyzed by the spliceosome. A highly dynamic, macromolecular RNP complex is formed out of the U1, U2, U4, U5 and U6 snRNPs and multiple snRNP proteins. During the early stages of spliceosome assembly, ... -
The roles of RNA helicases and other ribosome biogenesis factors during small subunit maturation
(2020-05-29)RNA helicases are a highly conserved family of proteins that act as RNA-dependent NTPases. These proteins contain a conserved helicase core consisting of two RecA-like domains that are responsible for unwinding or annealing ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ...