Browsing Zentren & Graduiertenschulen by Advisor & Referee "Rehling, Peter Prof. Dr."
Now showing items 1-20 of 47
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Sarcomere signaling in a patient-specific iPSC model of hypertrophic cardiomyopathy
(2025-03-07)Hypertrophic cardiomyopathy (HCM) is a severe cardiac disorder associated with ventricular hypertrophy, diastolic dysfunction, and arrhythmias, which can lead to heart failure. Inherited forms of HCM are frequently caused ... -
Molecular mechanisms and cellular functions of RNA methyltransferases targeting non-coding RNAs
(2024-12-23)Cellular RNAs contain diverse modified nucleotides that regulate their structure, stability, biogenesis and molecular functions. In abundant non-coding RNAs such as ribosomal (r)RNAs, transfer (t)RNAs and small nuclear ... -
PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Phospholamban – Characterization of the Potential Interaction Partner SLMAP
(2024-10-10)The identification of novel PLN interaction partners is of high importance to understand how it is regulated on a molecular level and to develop new therapeutic strategies for cardiomyopathy. SLMAP, a novel putative PLN ... -
Structural and functional analyses of the HECT E3 ligase HACE1
(2024-04-19)Many crucial cellular processes rely on post-translational modifications. In particular, the covalent attachment of ubiquitin molecules, termed ubiquitylation, onto substrates, typically via lysine residues, is most commonly ... -
Functions of cytoplasmic lattices in mammalian oocytes and embryos
(2023-12-12)Preimplantation embryogenesis is dependent on maternally stored proteins, transcripts, and other macromolecules. Although this is a well-established dogma, it is unclear how mammalian oocytes store maternal factors needed ... -
Mechanism of non-canonical ubiquitination by ubiquitin conjugating enzyme Ubc6
(2023-10-18)Protein quality control by ubiquitination occurs due to the sequential action of three enzyme classes: ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligases (E3). Conventionally, ubiquitin (Ub) ... -
Monitoring mitochondrial translation with spatial resolution and high throughput strategies
(2023-05-30)Nearly all mitochondrial proteins are encoded in the nuclear genome, synthesized in the cytosol, and imported into mitochondria. However, mitochondria are semi-autonomous and possess their own genome, the mitochondrial DNA ... -
Mitochondrial adaptation at the neuronal presynapse
(2021-11-22)Synaptic transmission poses a major energy consuming process in the brain, but how neurons maintain a constant energy supply during extended synaptic activity and how presynaptic mitochondria contribute to the energy ... -
Systematic Analysis of Molecular and Cellular Dysfunction in Accelerated Aging Phenotypes
(2021-09-24)Accelerated aging in multiple tissues is the connecting characteristic of the group of genetic disorders called segmental progeroid syndromes. At young ages, affected patients show many clinical features of aging-associated ... -
Investigation of Mitochondrial Ribosome Regulators at the Inner Membrane
(2021-09-24)Mitochondrial biogenesis relies on the proper assembly of proteins from dual genetic origin. Nuclear-encoded proteins are imported into the organelle and incorporated into multiprotein complexes together with proteins ... -
Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Reconstitution of Doa10-mediated ER-associated protein degradation with purified components
(2020-11-09)ER-associated protein degradation (ERAD) is a protein quality control pathway at the ER membrane mediated by membrane-embedded ubiquitin ligase complexes. During ERAD, membrane and luminal proteins are ubiquitinated and ... -
Insights into mitochondrial presequence and carrier import pathways
(2020-10-20)The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential ... -
Characterization of Mitochondrial Calcium Uniporter in Barth Syndrome Models
(2020-07-15)The Barth Syndrome (BTHS) is caused by the loss of the mitochondrial acyltransferase tafazzin, which is involved in the remodeling process of the hallmark mitochondrial glycerophospholipid cardiolipin. Cardiolipin maintains ... -
Optimization of tools for multiplexed super resolution imaging of the synapse
(2020-07-09)The synapse is the major site of neurotransmission in the brain. Even though the synapse has been extensively studied, artefact-free imaging tools are still necessary for its correct investigation. With the resolution of ... -
Reconstitution of retrotranslocation by the Hrd1 ubiquitin ligase with purified components
(2020-03-11)In eukaryotic organisms, a large fraction of newly-synthesized proteins are destined for the endomembrane system or for secretion. The entry gate for these proteins is the endoplasmic reticulum (ER). Proteins are imported ... -
Analysis of translational fidelity in cellular proteins
(2019-03-27)The accuracy of protein biosynthesis determines the quality of the proteome and regulates the fitness of the cell. A comprehensive quantification of miscoding events in the cell is essential to understand the basic mechanisms ... -
Analysis of mitochondrial transcription and replication on the single nucleoid level
(2019-03-13)Human mitochondrial DNA (mtDNA) is a circular molecule encoding for 13 subunits of the respiratory chain that are essential for its maintenance. Inside the mitochondria, mtDNA is associated with various proteins, forming ...