Browsing Zentren & Graduiertenschulen by Advisor & Referee "Rodnina, Marina Prof. Dr."
Now showing items 1-20 of 45
-
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Role of internal mRNA modifications in modulating translation elongation
(2024-09-26)Translation is a highly regulated process that defines the composition of the cellular proteome. Numerous mechanisms are known to fine-tune the translational output at different cellular conditions. Recently, a new regulatory ... -
Regulation of human 48S initiation complex formation
(2024-04-23)During the initiation phase of protein synthesis in humans, the small ribosome subunit interacts with initiator tRNA (tRNAi) and at least ten different initiation factors (eIFs) to form the initiation complex. The initiation ... -
Elongation factor P: mechanism of action and opportunities for drug design
(2023-07-24)EF-P is a conserved protein that has shape similarity to the tRNAs and binds to the E site of the ribosome. EF-P enhances the rate of peptide bond formation during the synthesis of polyPro sequences. In E. coli one third ... -
Release of a Natural mRNA During Recycling of the Prokaryotic Ribosome
(2023-06-20)Ribosome recycling is the last step of the bacterial translation cycle. The release of the peptide leaves a 70S ribosome with a deacylated tRNA in the P site and the stop codon of the mRNA in the A site. The combined action ... -
Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
Cotranslational folding of HemK C-terminal domain
(2023-03-17)The correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and ... -
Co-translational processing and membrane insertion of proteins in bacteria
(2022-11-04)Protein biogenesis entails the synthesis of polypeptides on the ribosome, their co- and post-translational maturation, folding, and, in the case of membrane proteins, insertion into the lipid bilayer. Bacterial proteins ... -
Ribosome dynamics during spontaneous frameshifting
(2022-10-18)During protein synthesis, the ribosome moves along the mRNA in steps of three nucleotides at a time to maintain the translational reading frame. However, on some mRNA sequences that are denoted as slippery, ribosomes may ... -
Kinetic analysis of neuronal SNARE protein interactions
(2022-08-08)When an action potential arrives at the axon terminal, it causes a local and transient increase in the Ca2+ concentration that starts a cascade of molecular interactions eventually leading to the fusion of synaptic vesicles ... -
Structure-function analysis of human nucleotide excision DNA repair
(2021-04-09)An average human cell accumulates tens of thousands of DNA lesions per day. Therefore, DNA repair pathways are set up to continuously proofread the DNA and correct DNA lesions, thus ensuring accurate expression and ... -
Kinetic Analysis of Mammalian Translation Initiation
(2021-01-22)During translation initiation in mammalian cells, 80S ribosomes are assembled at the translation start site on mRNA via a cap-dependent scanning mechanism. The formation of the 80S initiation complex (IC) is essential for ... -
Insights into the biogenesis of the human mitochondrial ribosomal large subunit – Characterisation of mL44 and mL45
(2020-11-16)Due to the presence of high-resolution cryo-EM structures of the human mitoribosome, the understanding about the function and assembly of the intriguing mitochondrial ribonucleoprotein complex increased tremendously during ... -
Development, characterization, and application of RNA catalysts for in situ labeling of target RNA molecules
(2020-08-07)RNA molecules play a diverse set of crucial roles in biological systems. Studying various aspects of RNA biology such as RNA transport, localization, degradation, and structural dynamics is therefore of utmost significance. ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Mechanism of Ribosome Rescue by Alternative Release Factor B
(2020-07-09)Rescue of ribosomes stalled on non-stop mRNAs is essential in bacteria and in the mitochondria of all eukaryotes. The lack of a stop codon causes ribosomes to stall at the 3’ end of mRNA with an empty A site, and specialized ... -
Monitoring dynamics of protein nascent chain on the ribosome using PET-FCS
(2020-06-24)The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold ... -
Programmed Translational Readthrough in Drosophila melanogaster
(2020-06-05)Translational readthrough (TR) is extensively used by viruses to expand their limited genome capacity. The pervasiveness of TR across eukaryotic genomes is only recently being explored. Systems biology approaches such as ... -
Functional analyses of RNA helicases in human ribosome biogenesis
(2019-10-30)Eukaryotic ribosomes are essential ribonucleoprotein (RNP) complexes consisting of four ribosomal RNAs (18S, 5S, 5.8S, 28S/25S) and approximately 80 ribosomal proteins, which are required for the process of translating the ... -
The Role of EF-G in Translational Reading Frame Maintenance on the Ribosome
(2019-09-10)Translation of an mRNA by the ribosome is the final step of gene expression. During translation initiation, the ribosome establishes the mRNA reading frame with the help of initiator tRNA binding to the start codon. This ...