Browsing Zentren & Graduiertenschulen by Advisor & Referee "Zweckstetter, Markus Prof. Dr."
Now showing items 1-20 of 27
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Molecular dynamics simulations of ion permeation and gating in potassium channels
(2025-03-13)Potassium (K+) channels are transmembrane ion channels that conduct K+ ions with exceptional selectivity and permeation rates. K+ channels participate in a plethora of important cellular processes, including termination ... -
Formation, regulation and dynamics of phase separated postsynaptic density condensates with Tau and Fyn
(2024-11-22)Alzheimer’s disease is characterized by memory loss and cognitive impairments. The cognitive deficits and synaptic dysfunction are associated with the excitotoxicity mediated by NMDA (N-methyl-D-aspartate) receptors on the ... -
Structural insight of MODAG-005 on 𝛼-Synuclein aggregates as a novel PET tracer
(2024-11-15)Neurodegenerative disorders, including Parkinson's disease (PD) and related synucleinopathies, are characterized by the accumulation of pathological protein aggregates in the brain. Among the key proteins implicated in ... -
Structural studies of RNA polymerase II complexes with human TFIIS and capping enzymes
(2023-12-14)Transcription is a conserved fundamental cellular process carried out by RNA polymerases (Pol) that results in the synthesis of an RNA transcript. Transcription of protein coding genes by Pol II is the first step in ... -
Exploring the human Hsp70-Hop-Hsp90 system for Tau recognition
(2022-03-22)Molecular chaperones are diligent helpers of the cell that ensure a well-balanced proteome by regulating protein production, protein folding, refolding and holding as well as protein degradation. In doing so, molecular ... -
Systematic comparison of the molecular mechanisms underlying the spreading of pathology in different neurodegenerative diseases
(2021-10-19)Misfolding and accumulation of disease-related proteins are common hallmarks among several neurodegenerative diseases. This phenomenon causes the progressive loss of cognitive and motor functions correlated with specific ... -
α-synuclein disrupts neuron network rhythmic activity when overexpressed in cultured neurons
(2021-03-09)Synucleinopathies are associated with a generalized slowing of the brain’s rhythmic activity, which co-occurs with dementia and has been connected with increased levels of α synuclein in both patients and test animals. In ... -
Investigating phase separation mechanisms for transcriptional control
(2020-11-13)Transcription of protein-coding genes by RNA polymerase (Pol) II is a highly coordinated process. In metazoan cells, transcription is regulated both at the initiation step by recruitment of the Pol II machinery as well as ... -
The Mediator kinase module: structural and functional studies in transcription regulation
(2020-10-15)Transcription of protein-coding genes occurs when RNA polymerase II (RNAPII) and general transcription factors (GTFs) assemble at genomic promoter regions, forming a transcription pre-initiation complex (PIC), which ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
Optimization and application of Trim-Away for studying a liquid-like spindle domain in mammalian oocytes
(2019-10-01)In this thesis, I reported a membraneless structure that permeated a major region of the female meiotic spindle and formed droplet-like protrusions around the spindle poles. Proteins within this previously unknown structure ... -
Pharmacologically controlled neurotrophic factor gene therapy for Parkinson's disease
(2019-07-24)During the PhD thesis, two projects were carried out. In the first project, an inducible AAV-5 vector for expression of GDNF, under control of the approved small molecule drug mifepristone, was evaluated in rats. GDNF ... -
Neurotoxicity and aggregation of β-synuclein and its P123H and V70M mutants associated with dementia with Lewy bodies
(2019-06-14)Dementia with Lewy bodies is a progressive degenerative brain disorder which accounts for 7 to 25% of all dementia cases. Two rare missense mutations of β-synuclein, P123H and V70M were identified in familial and sporadic ... -
Structure-based mechanistic analysis of the proteasome
(2019-05-02)The ubiquitin proteasome pathway is one of the major protein degradation pathways in the cell. This pathway is essential for protein quality control and the regulation of the cell cycle. Proteins subjected to the ubiquitin ... -
Molecular insights into the Tau-actin interaction
(2018-05-04)Tau protein is classically considered as a neuronal microtubule-associated protein that stabilizes microtubules and supports the outgrowth of axons. The protein can modulate the transport of vesicles and organelles along ... -
Fullerene-Nitroxide Derivatives as Potential Polarizers for Dynamic Nuclear Polarization (DNP) in Liquid State
(2016-09-12)Nuclear magnetic resonance spectroscopy and imaging (NMR/MRI) are widely applied methods for non-destructive analysis of biological systems, materials and medical applications. Dynamic nuclear polarization (DNP) is a method ... -
Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics
(2016-01-26)Specific recognition between biomolecules is at the basis of all biological processes, and a mechanistic description of molecular recognition is crucial for a detailed understanding of these processes. Two limiting models ... -
NMR Spectroscopy of the Tau-Microtubule Interaction
(2015-12-10)Tau is an intrinsically disordered, microtubule associated protein and is responsible for the promotion of microtubule (MT) formation and stabilization. The human Tau protein has six different isoforms, which are expressed ... -
Paramagnetic tools for the structural analysis of high molecular weight proteins
(2015-12-10)Paramagnetic effects provide important structural and dynamic information of biomolecules. However, the attachment of lanthanides through small chelating organic molecules to proteins (the most common way to obtain ... -
The interplay between α-synuclein and Rab GTPases: Insights into the molecular basis of synucleinopathies
(2015-03-24)Ageing related diseases become of ever more significance with the demographic shift currently prevalent in society. Dementia and movement disorders impact individuals and families alike, as the progressive decline in ...