Browse by type "doctoralThesis"
Now showing items 1-20 of 1208
-
Structure, Mechanism and Regulation of human UMP Synthase
(2025-01-10)Enzymes are of critical importance for a large range of biochemical reactions, catalyzing the conversion of a substrate to a product by lowering the energy of the transition state. The resulting rate acceleration in ... -
Rate-limiting steps of autophagy initiation
(2024-12-23)Autophagy research has progressed tremendously since the identification of ‘ATG’ genes and proteins responsible for maintaining the process. A dissection of general functions of all proteins involved was possible. However, ... -
Characterization of VAPA at the inner nuclear membrane: localization, membrane insertion and nuclear interactome
(2024-12-19)Tail-anchored (TA) proteins are type IV single-pass membrane proteins characterized by a long hydrophobic transmembrane domain (TMD) positioned very close to a notably short C-terminus facing the lumen. Due to their topology, ... -
Investigation of the dual role of mL62 during the mitoribosome assembly and rescue
(2024-12-19)Mitochondria are eukaryotic organelles, which have their own DNA (mtDNA) that encode for 13 proteins that are essential for the oxidative phosphorylation. The mitochondrial ribosome (mitoribosome) is responsible for the ... -
The dynamics of singularities in passive, driven, and active matter
(2024-12-13)Out-of-equilibrium statistical physics studies the time evolution of large collections of particles in terms of a reduced number of meso- and macroscopic observables. Their nature emerge from of the underlying microscopic ... -
Transport mechanisms of the putative vesicular nucleotide transporter VNUT
(2024-12-13)The vesicular nucleotide transporter VNUT (SLC17A9) is thought to refill recycled synaptic vesicles and other secretory vesicles with adenosine triphosphate (ATP) for chemical signal transmission. ATP translocation by VNUT ... -
Mechanisms of chromosome segregation in mammalian oocytes
(2024-12-10)Errors in chromosome segregation in human females often result in eggs with the wrong number of chromosomes, a condition known as aneuploidy. The rate of aneuploidy increases dramatically in women with age, particularly ... -
Advancements in Nanobody Applications: From Live Synaptic Imaging to Multiplexed Fluorescence Microscopy and Nanoscale Topography Mapping
(2024-12-09)Fluorescence microscopy facilitates biological research by enabling straightforward visualization of fluorescently labeled proteins in live and fixed samples. Despite significant progress in microscopy techniques, including ... -
PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Formation, regulation and dynamics of phase separated postsynaptic density condensates with Tau and Fyn
(2024-11-22)Alzheimer’s disease is characterized by memory loss and cognitive impairments. The cognitive deficits and synaptic dysfunction are associated with the excitotoxicity mediated by NMDA (N-methyl-D-aspartate) receptors on the ... -
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Structural insight of MODAG-005 on 𝛼-Synuclein aggregates as a novel PET tracer
(2024-11-15)Neurodegenerative disorders, including Parkinson's disease (PD) and related synucleinopathies, are characterized by the accumulation of pathological protein aggregates in the brain. Among the key proteins implicated in ... -
Interplay of proteins and lipids during endoplasmic reticulum-associated protein degradation
(2024-11-14)The endoplasmic reticulum controls lipid homeostasis due to its central role in lipid biosynthesis and its regulation. Aberrant lipid composition and membrane properties in the endoplasmic reticulum interfere with its ... -
Generation of innervated engineered human myocardium (iEHM) from human induced pluripotent stem cells to elucidate the mechanism underlying neuro-cardiac pathologies
(2024-11-12)Brain and heart consist of networks of excitable cells, the function of which is orchestrated by a complex interplay of ionic currents. Mutations in ion channels can predispose patients to cardiocerebral channelopathies ... -
Cell death signaling in the Arabidopsis chitin receptor mutant cerk1-4
(2024-11-07)Plant surface immunity is mediated by pattern recognition receptors (PRRs) that recognize con-served molecules derived from microorganisms. In Arabidopsis, CERK1 is the main component of the receptor complex that binds ... -
Probing the function of Ca2+-binding C2 domains of otoferlin, a synaptic protein of sensory hair cells
(2024-11-05)The ribbon synapses in auditory inner hair cells (IHCs) are specialized to achieve indefatigable sound encoding at rates of hundreds of Hertz and with sub-millisecond temporal precision. While conventional synapses employ ... -
Deep Myocardial Phenotyping of the Noonan Syndrome-causing Mutation RIT1<sup>F82L/+</sup> In Vitro and RAS/MAPK Signalling as a Therapeutic Target
(2024-10-23)Noonan syndrome (NS) belongs to the RASopathies, which describe a group of rare developmental disorders caused by heterozygous germline mutations leading to a dysregulation of the Ras/mitogen-activated protein kinase ... -
CarotPhyte: Exploring the evolutionary roots for the biosynthesis of apocarotenoids and their role as signals in plastid-mediated stress response in streptophyte algae
(2024-10-18)Plant terrestrialization came with enormous multidimensional consequences that shaped planet earth. This terraforming event that occurred about 550 MYA goes back to a single progenitor. Elucidating important facilitators ... -
Depletion of respiratory chain complexes modulates cristae architecture via MIC10 in human cells
(2024-10-18)The complex structure of the inner mitochondrial membrane plays a key role in mitochondrial function. The inner mitochondrial membrane is divided into the inner boundary membrane and the cristae membrane, which is characterized ... -
A light-inducible Na+ pump to study the role of intracellular Na+ accumulation in heart failure
(2024-10-10)Elevated Nai levels have been identified as a significant factor in the development of heart failure, observed across multiple species. This is caused by downregulated NKA, increased NHE activity and elevated late Na+ ...