Dokumente Fakultät für Biologie und Psychologie (inkl. GAUSS) nach Gutachter "Ficner, Ralf Prof. Dr."
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A method for the genetically encoded incorporation of FRET pairs into proteins
(2014-09-03)Proteins are composed of 20 canonical amino acids whose unique arrangements predefine a protein’s structure and function. Importantly, most proteins are not static conformations but rather very dynamic entities that undergo ... -
A novel rRNA quality control function for Npl3 in Saccharomyces cerevisiae
(2024-03-25)In all domains of life, ribosomes are essential complexes that are the sole source for protein synthesis. Ribosome biosynthesis, the most energy-consuming process in a cell, has to be tightly regulated. Misregulation ... -
A Role for Glycogen Synthase Kinase 3 beta in the Regulation of Glucagon Gene Transcription by Insulin
(2003-11-21)Insulin binding to its receptor on the a-cells in endocrine pancreas initiates a signalling cascade that results in inhibition of glucagon gene transcription. Evidence suggests that the ... -
Alterations in gene expression and secondary metabolite production during development of Aspergillus nidulans
(2014-02-10)Many studies describing development and secondary metabolism of the filamentous fungus Aspergillus nidulans contributed to a better understanding of fungal secondary metabolism regulation at the molecular level. However, ... -
Analyses of the Life Cycle of TLC1 and the Nuclear RNA Quality Control System in Saccharomyces cerevisiae
(2015-04-24)During genome replication, chromosomes undergo a progressive shortening of their ends. To counteract this loss, a complex named telomerase functions as a reverse transcriptase to elongate telomeres. In Saccharomyces ... -
Analysis of CRM1- and Nup214- dependent nuclear export of proteins
(2012-12-12)Nuclear pore complexes (NPCs) are giant units of the nuclear envelope, mediating directional transport into and out of the nucleus. Nucleoporins are the main components of the NPC, and one third of them contain ... -
Biochemical and cell biological analysis of the mechanism of RNA interference in human cells
(2006-04-10)RNA interference (RNAi) in human cells is efficiently triggered by short interfering RNA (siRNA) duplexes of 19-24 base-pairs (bp), which mimic the double-stranded processing intermediates ... -
Biochemical and structural characterization of spliceosomes purified at defined stages of assembly from the yeast S. cerevisiae
(2013-09-06)Splicing of pre messenger RNA (pre mRNA) is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) comprising several small nuclear (sn) RNPs and numerous proteins. The spliceosome assembles on its pre ... -
Biochemical studies of the synaptic protein otoferlin
(2015-08-27)The C2 domain containing protein otoferlin is required for a late step in exocytosis from auditory inner hair cells and was hypothesized to act as a synaptic Ca2+ sensor. More roles for otoferlin in hair cell synaptic ... -
Biochemische und zellbiologische Untersuchungen zur Rolle der Cajal Bodies bei der Zusammenlagerung spleißosomaler UsnRNP Partikel
(2005-06-27)Eukaryotic pre-mRNA splicing is catalysed by a large macromolecular complex called the spliceosome. One central unit of the spliceosome is the [U4/U6.U5] tri-snRNP particle that consists ... -
Biophysical Characterization of SNARE Complex Disassembly Catalyzed by NSF and alphaSNAP
(2008-09-17)SNAREs (SNAP receptors) are small membrane-bound proteins which are key mediators of intracellular membrane fusion. The actual fusion step is believed to occur through complex formation of ... -
Biophysikalische und thermodynamische Charakterisierung der neuronalen SNARE-Komplexbildung
(2008-07-16)Membrane fusion events are mediated by a set of conserved protein machinery, the SNARE proteins. SNARE proteins assemble into a very stable α-helical complex between two membranes. The ... -
Carbon Catabolism in <i>Bacillus subtilis</i>: Global and Molecular Views on the Control of Gene Expression
(2007-11-12)The soil bacterium B. subtilis is exposed to frequently changing environmental conditions. Moreover, in its natural habitat, B. subtilis is surrounded by numerous microorganisms ... -
Characterizing the RanGAP1-RanBP2 complex in mitosis
(2009-03-18)The RanGAP1-RanBP2 complex represents a fascinating macromolecular assembly comprising at least two enzymatic activities. On one hand, it harbors the GTP hydrolysis activating function of ... -
Crystal structure analysis of selenocysteine biosynthesis components
(2010-11-04)Some organisms from all three domains of life eukaryotes, bacteria and archaea, incorporate selenocysteine (Sec) into fractions of their proteins. Sec synthesis takes place on its ... -
Crystallographic studies on diheme cytochrome <i>c</i> enzymes
(2007-11-30)Bacteria express various electron transport chains depending on their environment and their physiological state. Membrane electron transport systems carry electrons from a number of different ... -
Deneddylation and fungal development - Regulation of Nedd8 protein modification by DenA and the COP9 signalosome
(2012-08-16)Ubiquitin dependent protein degradation is a common principle in eukaryotic organisms to control appropriate temporal and spatial protein levels. Abnormal regulation leads to embryonic lethality in plants, insects and ... -
Determination of the Structure of the Spliceosomal U6 snRNP from Yeast, <i>Saccharomyces cerevisiae</i>
(2007-08-09)The U6 small nuclear RNA (snRNA) undergoes major conformational changes during the assembly of the spliceosome and catalysis of splicing. It associates with the specific protein Prp24p, ... -
Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP
(2008-10-30)Pre-mRNA splicing is catalyzed by a macromolecular machine called spliceosome . The spliceosome is assembled in a stepwise manner from the small nuclear ribonucleoprotein particles (snRNPs), ... -
Entwicklung von Substraten und Inhibitoren pharmakologisch relevanter Proteintargets
(2005-11-14)Thrombin: A series of thrombin inhibitors were generated by using powerful computer-assisted multiparameter optimization process. The process was organized in design cycles, ...