Browsing Fakultät für Biologie und Psychologie (inkl. GAUSS) by Referee "Lührmann, Reinhard Prof. Dr."
Now showing items 1-8 of 8
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Biochemical and structural characterization of spliceosomes purified at defined stages of assembly from the yeast S. cerevisiae
(2013-09-06)Splicing of pre messenger RNA (pre mRNA) is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) comprising several small nuclear (sn) RNPs and numerous proteins. The spliceosome assembles on its pre ... -
Investigation of the structure of spliceosomal complexes from the yeast S. cerevisiae
(2021-03-19)Nuclear pre-mRNA splicing is catalysed by the spliceosome, a multi-megadalton ribonucleoprotein (RNP) complex. It assembles de novo for each round of splicing on premRNA intron with stepwise binding of five small nuclear ... -
Isolation and characterization of tissue and development-specific microRNAs from Drosophila, mouse and human
(2005-07-19)MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression in plants, animals and viruses. These small RNAs provide specificity to protein effector complexes mediating ... -
Structural and functional analysis of exportin-cargo recognition
(2010-11-19)All nucleocytoplasmic exchange proceeds through nuclear pore complexes, which constitute giant aqueous channels in the nuclear envelope. Most of this traffic is mediated by Ran-dependent ... -
Structural characterization of human spliceosome activation by cryo-EM
(2022-01-27)Eukaryotic genes are transcribed as precursor mRNA (pre-mRNA), in which coding regions (exons) are interrupted by non-coding regions (introns). Introns are excised and exons are ligated together in a two-step process ... -
The function of Nup358 in nucleocytoplasmic transport
(2010-10-20)Nucleocytoplasmic transport occurs through nuclear pore complexes (NPC). Some nucleoporins, components of the NPC, have been suggested to function as initial or terminal binding sites for ... -
Transcriptome maps of general eukaryotic RNA degradation factors and identification and functional characterization of the novel mRNA modification N<sup>3</sup>-methylcytidine
(2019-07-01)The first part of this dissertation focused on the different RNA degradation pathways in the yeast Saccharomyces cerevisiae (S. cerevisiae). We used the method photoactivatable ribonucleoside-enhanced crosslinking and ... -
Untersuchungen zum Mechanismus der katalytischen Aktivierung von Spleißosomen aus Saccharomyces Cerevisiae
(2013-02-13)Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) complex. It assembles anew on each pre-mRNA intron by the stepwise binding of five snRNPs (U1, U2, U4, U5 and U6) and numerous ...