Browsing Fakultät für Chemie (inkl. GAUSS) by Advisor & Referee "Griesinger, Christian Prof. Dr."
Now showing items 1-20 of 23
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Detecting the Undetectable: Functional Protein Motions in the Hidden Timescale Window Revealed by NMR Relaxation Measurements
(2022-05-19)About 50 years ago, even before NMR was ready to determine structure of proteins, it could unveil a surprising motion in aromatic side-chain (Wuthrich & Wagner, 1975) from 1D 1H spectra. With decades of development, NMR ... -
In and through the membrane by solid-state NMR: investigations of the human voltage-dependent anion channel 1 and Nup98-derived FG-domains
(2022-02-22)Magic-angle-spinning NMR spectroscopy is a powerful tool for the investigation of protein structure, dynamics and interactions at atomic resolution. We have applied this technique to investigate two widely different systems: ... -
Revealing atomic resolution structural insights into membrane proteins in near-native environments by proton detected solid-state NMR
(2021-05-18)Membrane proteins are an essential part of any living organism. They are cellular switches that trigger metabolic cascades regulating the cell metabolism, including cell division, gene expression and others functions as ... -
Oligomer modulator anle138b and related compounds in neurodegeneration and beyond
(2020-12-10)Neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, Creutzfeldt-Jakob disease are characterized by a progressive deterioration of brain function as a result of a gradual loss of nerve cells. Due ... -
Enhancement Strategies in NMR Spectroscopy
(2018-05-14)Hyperpolarization methods have become a powerful assistance technique to NMR spectroscopy in order to improve its sensitivity and consequently reduce the required measurement time to achieve a satisfactory signal-to-noise ... -
Synthese chiraler Alignmentmedien zur Enantiomerenunterscheidung via anisotroper NMR-Parameter & Bestimmung der absoluten Konfiguration von (–)-erythro-Mefloquin HCl
(2014-08-12)During this PhD project it was presented a new chiral alignment medium for the enantiodiscrimination of chiral amines. Initially (R)- or (S)-2-acrylamido-2-phenylethane sulfonic acid, respectively ((R)- / (S)-APhES), the ... -
Thermal coefficients of methyl groups within ubiquitin and metabolic coupling of NAA and lactate in cortical neurons
(2013-10-30)Protein folding and molecular recognition are physiological processes that are intricately linked to their dynamic signature and reflected in their thermal coefficients. Additionally, the local conformational entropy is ... -
Development and application of NMR methods for challenges in drug discovery
(2013-05-03)NMR spectroscopy plays an important role in all stages of the drug discovery process. Namely in the structure elucidation of natural products, synthetic ligands and metabolites, as well as a high-throughput screening ... -
Stereochemistry of Challenging Natural Products Studied by NMR-based Methods
(2013-02-18)Stereochemistry is a very important subject in chemistry and life sciences, as most of the biologically active molecules, including proteins, oligonucleotides, sugars, lipids and small molecules exist in one chiral form. ... -
Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43
(2012-11-19)Connexin 43 (Cx43) is the most prominent member of the connexin family in mammalian tissues. In biological lipid membranes, six connexin subunits assemble into a hemichannel (connexon). Direct communication of apposing ... -
Solid-state NMR characterization of Alzheimer-like tau amyloid fibrils
(2012-09-27)One of the hallmarks of Alzheimer's disease (AD) is the self-assembly of the microtubule-associated protein tau into fibers termed “paired helical filaments -
Entwicklung von Lanthanoid-Tags für die biomolekulare NMR-Spektroskopie
(2011-05-02)Paramagnetically induced effects such as pseudocontact shifts (PCSs) and residual dipolar couplings (RDCs) are valuable parameters for biomolecular NMR spectroscopy. They contain angular ... -
Integration von Connexonen in Lipidmembranen auf porösen Oberflächen
(2010-08-18)Connexin26 (Cx26) is a member of the connexin family, the building blocks for gap junction intercellular channels. These dodecameric assemblies are involved in gap junction-mediated ... -
Paramagnetisch markierte Oligonukleotide
(2009-09-11)Two methods to obtain paramagnetically tagged oligonucleotides are described. To get a biomolecule with a paramagnetic center a radical or metal moiety has to be introduced. This was done ... -
Transition of intrinsically unfolded α-synuclein into the fibrillar state characterized by NMR spectroscopy
(2008-12-10)Proteins fold into appropriate configuration, called native structure, in order to achieve its cellular function. A protein with nonnative structure induces malfunction and causes the ... -
Characterization of the amyloid precursor of alpha-synuclein by NMR spectroscopy
(2008-11-04)A number of human neurodegenerative diseases, including Alzheimer"s and Parkinson"s diseases (PD), are associated with the deposition of proteinaceous aggregates known as amyloid fibrils. ... -
NMR investigations of 15.5K associated protein-RNA complexes
(2008-01-28)The spliceosome, consisting of different snRNPs and numerous non-U snRNP factors, catalyzes the splicing reaction. To activate the spliceosome and enable the first step of splicing to begin, ... -
Strukturelle und funktionelle Charakterisierung von dem mitochondrialen Membranprotein Menschlicher Spannungsabhängiger Anionen Kanal (HVDAC) und dem Membranprotein bindenden Conotoxin Conkunitzin-S1 mit Flüssigphasen NMR
(2007-08-02)This thesis sheds light on the mode of action of membrane proteins and their ligands. Two model systems were chosen: the membrane protein was the human voltage dependent anion channel (HVDAC) ... -
Development and Application of NMR-methods for Structural Investigations of Small Molecules and Proteins
(2007-05-29)The elucidation of the relative stereochemistry of asymmetric centers of organic molecules is an important challenge in chemistry since it requires the simultaneous determination of ... -
TAR-RNA Recognition by a Novel Cyclic Aminoglycoside Analogue
(2007-05-29)The formation of the Tat-protein/TAR RNA complex is a crucial step in the regulation of Human Immunodeficiency Virus (HIV)-gene expression. To obtain fulllength viral transcripts the Tat/TAR ...