Browsing Fakultät für Chemie (inkl. GAUSS) by Advisor "Steinem, Claudia Prof. Dr."
Now showing items 1-20 of 31
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Aufbau einer Silicanin-1-haltigen Modellmembran des Silicafällungsvesikels aus Thalassiosira pseudonana
(2021-12-23)Silica polycondensation occurring in diatom organelles called silica deposition vesicles (SDVs) leads to valve and girdle band formation to complete the biosilica cell wall after cell division. This biosilica shows ... -
Die Biomineralisation von Silica: Langkettige Polyamine und Aminolipide als selbstorganisierende Template für biomimetische Präzipitationen
(2010-02-09)Long-chain polyamines are thought to play an important role in the formation of the hierarchically structured silica cell walls of diatoms. We determined the influence of structural variations ... -
Charakterisierung der Aktivität und Inhibition des rekombinanten, spannungsgesteuerten Protonenkanals HV1: Funktionelle Rekonstitution in unilamellare Vesikel
(2018-10-15)The human voltage-gated proton channel (hHV1) regulates important physiological processes like the respiratory burst activity of white blood cells and the motility of spermatozoa by efflux of protons. Reconstitution of the ... -
Directing macromolecular assemblies by tailored surface functionalizations of nanoporous alumina
(2011-05-19)Anodic aluminum oxide (AAO) is a nanoporous material with parallel, hexagonally-ordered, cylindrical pores running straight through the material s thickness. A method to deposit highly ... -
Dynamische Strukturen am Zellcortex: Aktivierbarkeit und Akkumulation von Ezrin in Abhängigkeit von PIP2
(2011-03-23)Ezrin, a member of the ezrin/radixin/moesin (ERM) protein family, acts as a membrane-cytoskeleton linker, thereby participating in a variety of cellular events. The N-terminal region of ... -
Einfluss des Zellkortex auf die Plasmamembran: Modulation von Mikrodomänen in Modellmembranen
(2013-03-14)The architecture of the plasma membrane is not only determined by the lipid and protein composition but is also influenced by its attachment to the underlying cytoskeleton. The aim of this thesis was to create a new model ... -
Elektrophysiologische Charakterisierung des mitochondrialen Porins VDAC1 und des antimikrobiellen Peptids Dermcidin in lösungsmittelfreien Modellmembranen
(2013-07-04)Solvent free lipid bilayers were used for the electrophysiological characterization of the most abundant protein of the outer mitochondrial membrane, the isoform 1 of the voltage dependent anion channel (VDAC1), and the ... -
Entwicklung eines Fusionsassays basierend auf porenüberspannenden Membranen
(2011-11-09)Fusion of biological membranes is a central requirement for many cellular processes. In recent years a great variety of fusion assays based on artificial membrane systems has been developed. ... -
Establishment of a fluorescence assay for characterization of protein-mediated vesicle fusion and acidification
(2017-06-21)Membrane proteins mediate a manifold of essential transport processes across the lipid membrane of cells and organelles, therewith representing important drug targets. To investigate protein-mediated transport in vitro, ... -
Ezrin activation in vitro: Investigation of ezrin's conformation and the interaction between ezrin and F-actin
(2013-12-19)The function of ezrin, a member of the ezrin-radixin-moesin (ERM) protein family, is to regulate the cell membrane architecture within the context of fundamental biological processes by linking the membrane and the actin ... -
Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43
(2012-11-19)Connexin 43 (Cx43) is the most prominent member of the connexin family in mammalian tissues. In biological lipid membranes, six connexin subunits assemble into a hemichannel (connexon). Direct communication of apposing ... -
HIV-1 Nef destabilisiert artifizielle Membransysteme: Untersuchung der Bedeutung des Myristoylankers und des positiven Ladungsclusters
(2009-06-22)The HIV-1 Nef protein belongs to the so-called accessory proteins and is critical for optimal viral replication and the pathogenesis of AIDS. It is supposed that the functions of Nef require ... -
Hochohmige porenüberspannende Lipidmembranen: Elektrochemische Untersuchungen zur Aktivität von Gramicidin und Bacteriorhodpsin
(2009-07-14)To study transmembrane proteins they need to be integrated in a lipid environment to preserve their functionality. For this purpose, model membranes have been developed which allow the ... -
In vitro studies of protein interactions on substrate supported artificial membranes
(2013-02-12)Protein interactions that take place at the interface of a membrane are abundant within cellular organisms. Hence, surface coupled protein interactions are a matter of ongoing scientific investigation. In this work, elected ... -
Influence of ezrin's actin binding properties on the formation of minimal actin cortices
(2024-02-16)The cytoskeleton is a pivotal structure within cells, crucial for their motion and life-indispensable processes. The actin cortex is a dense actin network that is part of the cytoskeleton and linked to the plasmamembrane. ... -
Influence of point mutations on the electrophysiological properties of a bacterial porin and its interaction with a β-lactam antibiotic
(2018-09-19)The high rate of complications and mortality in hospitals due to multidrug resistances (MDR) of gram-negative bacteria demonstrates the urgency to understand the underlying mecha-nisms. One common cause of MDR are mutations ... -
Integration von Connexonen in Lipidmembranen auf porösen Oberflächen
(2010-08-18)Connexin26 (Cx26) is a member of the connexin family, the building blocks for gap junction intercellular channels. These dodecameric assemblies are involved in gap junction-mediated ... -
Interaction of antimicrobial compounds with lipid bilayers in established and novel membrane model systems
(2021-05-06)Investigating the interaction between antimicrobial compounds and membrane model systems in the form of artificial lipid bilayers provides important insights into their mode of action. The interaction of lipid side chains ... -
Interaction of he Epsin N-Terminal Homology domain (ENTH) with artificial membranes as a function of lateral tension
(2016-09-01)During endocytosis, membrane remodeling of a planar membrane into a highly curved vesicle is controlled by a complex protein machinery. However, the regulatory role of physical properties such as membrane tension is ... -
Investigating Synthetic Approaches of the Neuroregulator α-Synuclein
(2024-01-04)Neuronal disorders such as Parkinson's disease, multiple system atrophy and dementia with Lewy bodies affect millions of patients worldwide. They belong to a group of neurodegenerative diseases called synucleinopathies, ...