Browsing Naturwissenschaften, Mathematik und Informatik by Advisor "Jakobs, Stefan Prof. Dr."
Now showing items 1-10 of 10
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Engineering of a bacteriophytochrome-derived reversibly switchable fluorescent protein for the application in super-resolution microscopy in the near-infrared window
(2022-06-29)The developments of fluorescence microscopy techniques has advanced our understanding of biological systems on a cellular level. In recent years, several super-resolution microscopy techniques overcame the diffraction ... -
Investigation of the mitochondrial contact site and cristae organizing system and its role in cristae formation
(2020-10-27)Mitochondria are essential organelles in eukaryotic cells that feature a unique doublemembrane architecture. The mitochondrial inner membrane forms numerous invaginations, named cristae, which can adopt different shapes ... -
One-step RESOLFT with a positively switchable RSFP with improved deactivation kinetics
(2020-10-01)Interaction and spatial organization of subcellular structures within living cells are an essential aspect of biological research, but conventional microscopy can elucidate these properties only to a finite extent as it is ... -
In vivo super-resolution live-cell RESOLFT-microscopy of Drosophila melanogaster and Arabidopsis thaliana
(2018-04-23)The optical resolution of conventional light microscopy is limited by the diffraction barrier of light. This resolution limit impedes the characterization of cellular structures featuring a size smaller than half the ... -
Gerichtete Evolution und Charakterisierung von Bakteriophytochromen für die tiefrote, hochauflösende Fluoreszenzmikroskopie
(2017-10-18)The green fluorescent protein (GFP) and its homologues have revolutionized molecular biology through their considerable utility as genetic probes, among myriad other applications. Moreover, in recent decades, the ... -
Etablierung eines Nachweisverfahrens zur Untersuchung der räumlichen und zeitlichen Verteilung mitochondrial translatierter Proteine mit hochauflösender STED-Mikroskopie durch metabolische Markierung mit nicht-kanonischen Aminosäuren
(2017-09-14)Metabolic labeling of nascent polypeptides using non-canonical amino acids is a valuable tool enabling researchers to introduce bioorthogonal functional groups into proteins. These can subsequentially be used for identifying ... -
Untersuchung der sub-mitochondrialen Proteinverteilung von MICOS mittels STED-Mikroskopie
(2015-09-28)Mitochondria are essential components of eukaryotic cells. They consist of an outer and inner membrane, which itself is divided into the inner boundary membrane and the cristae. The transition from the inner boundary ... -
Untersuchung der Heterogenität submitochondrialer Proteinverteilungen mit hochauflösender Mikroskopie
(2015-01-15)Mitochondria are essential organelles of eukaryotic cells. They fulfill a multitude of fundamental tasks: besides their outstanding significance for energy production they possess a central role in metabolism, ion homeostasis ... -
Untersuchung der sub-mitochondrialen Lokalisation des MINOS-Komplexes in humanen Zellen
(2014-05-28)MINOS (mitochondrial inner membrane organizing structure) is a large hetero-oligomeric complex in the inner mitochondrial membrane. Originally identified in mitochondria of yeast cells, components of the complex were found ... -
Generation of Novel Photochromic GFPs: Fluorescent Probes for RESOLFT-type Microscopy at Low Light Intensities
(2012-05-23)The resolution of a conventional light microscope is limited by diffraction. Hence, light microscopy failed to discern objects that are closer together than half the wavelength of light. STED (STimulated Emission ...