Browsing Naturwissenschaften, Mathematik und Informatik by Advisor "Steinem, Claudia Prof. Dr."
Now showing items 1-20 of 31
-
Influence of ezrin's actin binding properties on the formation of minimal actin cortices
(2024-02-16)The cytoskeleton is a pivotal structure within cells, crucial for their motion and life-indispensable processes. The actin cortex is a dense actin network that is part of the cytoskeleton and linked to the plasmamembrane. ... -
Investigating Synthetic Approaches of the Neuroregulator α-Synuclein
(2024-01-04)Neuronal disorders such as Parkinson's disease, multiple system atrophy and dementia with Lewy bodies affect millions of patients worldwide. They belong to a group of neurodegenerative diseases called synucleinopathies, ... -
Structure and Mechanics of Membrane-bound Vimentin Filaments and Networks under Strain
(2022-12-16)The shape and mechanical properties of eukaryotic cells are determined by the cytoskeleton, composed of actin filaments, microtubules and intermediate filaments (IFs). Among these, IFs are considered to be the main ... -
Reconstitution of Connexin-43 in Artificial Membranes
(2022-04-01)Synthetic biology aims to engineer complex biological functions in an artificial way. A variety of molecular tools and techniques allow mimicking of processes of living systems in a synthetic way. In that context, the ... -
Aufbau einer Silicanin-1-haltigen Modellmembran des Silicafällungsvesikels aus Thalassiosira pseudonana
(2021-12-23)Silica polycondensation occurring in diatom organelles called silica deposition vesicles (SDVs) leads to valve and girdle band formation to complete the biosilica cell wall after cell division. This biosilica shows ... -
Interaction of antimicrobial compounds with lipid bilayers in established and novel membrane model systems
(2021-05-06)Investigating the interaction between antimicrobial compounds and membrane model systems in the form of artificial lipid bilayers provides important insights into their mode of action. The interaction of lipid side chains ... -
Phase separation of biomimetic membranes:
(2019-12-11)The bacterium enterohemorrhagic Escherichia coli (EHEC) is one of the main causes of food borne illness. The toxin of this bacteria is shiga toxin (STx) which inhibits the protein biosynthesis of cells. Approaching the ... -
Charakterisierung der Aktivität und Inhibition des rekombinanten, spannungsgesteuerten Protonenkanals HV1: Funktionelle Rekonstitution in unilamellare Vesikel
(2018-10-15)The human voltage-gated proton channel (hHV1) regulates important physiological processes like the respiratory burst activity of white blood cells and the motility of spermatozoa by efflux of protons. Reconstitution of the ... -
Influence of point mutations on the electrophysiological properties of a bacterial porin and its interaction with a β-lactam antibiotic
(2018-09-19)The high rate of complications and mortality in hospitals due to multidrug resistances (MDR) of gram-negative bacteria demonstrates the urgency to understand the underlying mecha-nisms. One common cause of MDR are mutations ... -
Modulation of lateral membrane tension and SNARE-mediated single vesicle fusion on pore spanning membranes
(2017-09-25)Synaptic vesicle fusion is driven by soluble N-ethylmaleimide-sensitive- factor attachment receptors (SNAREs) but is also regulated by physical properties of the membrane like curvature and tension. Using artificial SNARE ... -
Solid Supported Model Membranes Containing Plant Glycolipids: A Tool to Study Interactions between Diatom Biomolecules and the Silicalemma in vitro
(2017-06-22)Silica biomineralization in diatoms is presumably controlled by a self-assembly of biomolecules inside membrane-enclosed silica deposition vesicles. The fine-patterned structure of the diatom’s cell wall is thereby determined ... -
Establishment of a fluorescence assay for characterization of protein-mediated vesicle fusion and acidification
(2017-06-21)Membrane proteins mediate a manifold of essential transport processes across the lipid membrane of cells and organelles, therewith representing important drug targets. To investigate protein-mediated transport in vitro, ... -
Verknüpfung zwischen Plasmamembran und Zytoskelett
(2016-09-22)The dynamic linkage between the plasma membrane and the underlying cytoskeleton of the cell is fundamental for cellular processes like cell morphogenesis, cell motility and cell adhesion. Ezrin as a member of the ERM ... -
Interaction of he Epsin N-Terminal Homology domain (ENTH) with artificial membranes as a function of lateral tension
(2016-09-01)During endocytosis, membrane remodeling of a planar membrane into a highly curved vesicle is controlled by a complex protein machinery. However, the regulatory role of physical properties such as membrane tension is ... -
Untersuchung einzelner SNARE-vermittelter Membranfusionsereignisse auf planaren porenüberspannenden Membranen
(2015-10-20)Even though there are a number of different in vitro fusion assays to analyze neuronal SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) mediated fusion, still the essential features of the in ... -
Spezifität der Wechselwirkung von Collybistin 2 mit Phosphatidylinositolphosphaten: Einfluss der verschiedenen Proteindomänen
(2015-05-27)Collybistin, a brain-specific GEF, is required for gephyrin clustering at the postsynaptic membrane. This is important for the correct arrangement of GABAA and glycine receptors. Binding of collybistin to a membrane is ... -
Ezrin activation in vitro: Investigation of ezrin's conformation and the interaction between ezrin and F-actin
(2013-12-19)The function of ezrin, a member of the ezrin-radixin-moesin (ERM) protein family, is to regulate the cell membrane architecture within the context of fundamental biological processes by linking the membrane and the actin ... -
Phospholipidmembranen auf mikroporösen Substraten: in situ Bildung elektrochemischer Gradienten
(2013-07-11)Electrochemical gradients are of utmost importance for cellular processes, such as the generation of action potentials or the production of ATP in mitochondria. They are established by membrane proteins, which consume ... -
Elektrophysiologische Charakterisierung des mitochondrialen Porins VDAC1 und des antimikrobiellen Peptids Dermcidin in lösungsmittelfreien Modellmembranen
(2013-07-04)Solvent free lipid bilayers were used for the electrophysiological characterization of the most abundant protein of the outer mitochondrial membrane, the isoform 1 of the voltage dependent anion channel (VDAC1), and the ... -
Einfluss des Zellkortex auf die Plasmamembran: Modulation von Mikrodomänen in Modellmembranen
(2013-03-14)The architecture of the plasma membrane is not only determined by the lipid and protein composition but is also influenced by its attachment to the underlying cytoskeleton. The aim of this thesis was to create a new model ...