Browsing Naturwissenschaften, Mathematik und Informatik by Referee "Griesinger, Christian Prof. Dr."
Now showing items 1-20 of 39
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Biophysical characterization and computational modeling of phase separation of immune adapter proteins
(2022-07-07)The processes of B cell activation and development are stimulated by antigen binding to the B cell receptor. Pre-signaling clusters are present in unstimulated, resting B cells and assemble by tripartite phase separation ... -
Detecting the Undetectable: Functional Protein Motions in the Hidden Timescale Window Revealed by NMR Relaxation Measurements
(2022-05-19)About 50 years ago, even before NMR was ready to determine structure of proteins, it could unveil a surprising motion in aromatic side-chain (Wuthrich & Wagner, 1975) from 1D 1H spectra. With decades of development, NMR ... -
In and through the membrane by solid-state NMR: investigations of the human voltage-dependent anion channel 1 and Nup98-derived FG-domains
(2022-02-22)Magic-angle-spinning NMR spectroscopy is a powerful tool for the investigation of protein structure, dynamics and interactions at atomic resolution. We have applied this technique to investigate two widely different systems: ... -
Structure and function of the membrane protein Opa60 by solid-state NMR
(2021-10-18)Studying the structure of transmembrane (TM) proteins is an important task since many of them are drug targets and are involved in essential cellular functions. However, new techniques are urgently needed, as TM proteins ... -
Revealing atomic resolution structural insights into membrane proteins in near-native environments by proton detected solid-state NMR
(2021-05-18)Membrane proteins are an essential part of any living organism. They are cellular switches that trigger metabolic cascades regulating the cell metabolism, including cell division, gene expression and others functions as ... -
Oligomer modulator anle138b and related compounds in neurodegeneration and beyond
(2020-12-10)Neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, Creutzfeldt-Jakob disease are characterized by a progressive deterioration of brain function as a result of a gradual loss of nerve cells. Due ... -
Effects of anle138b treatment on amyloid-β neurotoxicity
(2020-10-02)Alzheimer’s disease (AD), the leading etiology of dementia and the fifth cause of death worldwide, has been declared a public health priority by the World Health Organization in 2017. AD is incurable and no treatment can ... -
Studying the regulation and development of circadian clock by systems biology approaches
(2020-09-24)System biology approaches, coupled with emerging omics tools, have been widely used and greatly accelerated biological and medical research and discovery. In this thesis, I will focus on applying those tools and technologies ... -
Enhancement Strategies in NMR Spectroscopy
(2018-05-14)Hyperpolarization methods have become a powerful assistance technique to NMR spectroscopy in order to improve its sensitivity and consequently reduce the required measurement time to achieve a satisfactory signal-to-noise ... -
Modulating the aggregation of alpha-synuclein and prion protein with small molecules.
(2016-11-07)As living and health conditions improve in modern societies, the percentage of older people rises. A byproduct of this ageing is the marked increase of cases of cancer, cardiovascular, and neurodegenerative diseases. Many ... -
From Slow to Ultra-fast MAS: Structural Determination of Type-Three Secretion System Bacterial Needles and Inorganic Materials by Solid-State NMR
(2014-12-16)This work on solid-state NMR methodologies and applications is divided in three parts. In the first part, three methods for solid-state NMR at ultra-fast Magic-Angle Spinning (MAS) are introduced: completely low-power ... -
Synthese chiraler Alignmentmedien zur Enantiomerenunterscheidung via anisotroper NMR-Parameter & Bestimmung der absoluten Konfiguration von (–)-erythro-Mefloquin HCl
(2014-08-12)During this PhD project it was presented a new chiral alignment medium for the enantiodiscrimination of chiral amines. Initially (R)- or (S)-2-acrylamido-2-phenylethane sulfonic acid, respectively ((R)- / (S)-APhES), the ... -
Development and application of NMR methods for challenges in drug discovery
(2013-05-03)NMR spectroscopy plays an important role in all stages of the drug discovery process. Namely in the structure elucidation of natural products, synthetic ligands and metabolites, as well as a high-throughput screening ... -
Stereochemistry of Challenging Natural Products Studied by NMR-based Methods
(2013-02-18)Stereochemistry is a very important subject in chemistry and life sciences, as most of the biologically active molecules, including proteins, oligonucleotides, sugars, lipids and small molecules exist in one chiral form. ... -
Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43
(2012-11-19)Connexin 43 (Cx43) is the most prominent member of the connexin family in mammalian tissues. In biological lipid membranes, six connexin subunits assemble into a hemichannel (connexon). Direct communication of apposing ... -
Solid-state NMR characterization of Alzheimer-like tau amyloid fibrils
(2012-09-27)One of the hallmarks of Alzheimer's disease (AD) is the self-assembly of the microtubule-associated protein tau into fibers termed “paired helical filaments -
Structural and Funtional Studies on VitaminB1-Dependent Human and Bacterial Transketolases
(2012-08-24)Transketolases (TKs, EC 2.2.1.1) are ubiquitous enzymes in cellular carbohydrate metabolism that require the cofactor thiamin diphosphate for catalytic activity. TKs are involved in numerous metabolic pathways whereas the ... -
Dynamics and interactions of the voltage-dependent anion channel 1 studied by NMR spectroscopy
(2012-06-20)The voltage-dependent anion channel (VDAC), the most abundant protein in the outer mitochondrial membrane, acts as a gatekeeper for the entry and exit of mitochondrial metabolites and is involved in mitochondrial apoptosis. ... -
Understanding the molecular machinery of aquaporins through molecular dynamics simulations
(2012-01-02)Aquaporins are protein channels responsible for the permeation of water and other small solutes through biological membranes in response to osmotic pressure. The goal of the present thesis ... -
Correlated and Further Dynamics in Proteins by NMR Spectroscopy
(2011-09-27)In the first part of this thesis the use of three NMR parameter for the detection of protein dynamics are investigated. The knowledge about the dynamics of proteins is essential for the ...