Browsing Naturwissenschaften, Mathematik und Informatik by Referee "Tittmann, Kai Prof. Dr."
Now showing items 1-20 of 47
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The dual role of MTG3 during human mitoribosome biogenesis
(2024-12-05)Mitochondria are the main energy provider of the cell, and because of the endosymbiotic event they arise from, still contain a small independent genome. Only with the vast ATP production through the mitochondrial OXPHOS ... -
Characterization of the Phosphomimetic Mutant Y39E of a-Synuclein
(2024-08-06)α-Synuclein (aSyn) has long been identified as a key factor in the pathogenesis of Parkinson's Disease (PD), with familial mutations in the SNCA gene contributing to the aberrant aggregation of aSyn. Its aggregation results ... -
Macromolecules in Motion by High-resolution Cryo-EM
(2024-05-06)Protein synthesis, the translation of the genetic code into a defined sequence of amino acids, is a central, highly dynamic and regulated step in gene expression. The well-defined bacterial translation system enables to ... -
Probing SNARE-Induced Membrane Fusion Using Peptidic Model Fusogens
(2022-11-10)In this thesis, SNARE analogues with artificial recognition units made of either coiled-coil sequences or peptide nucleic acid (PNA) oligomers were synthesized. By applying FRET-based liposome fusion assays, the fusogenic ... -
Improving electron microscopic hardware to push the resolution limits in single particle cryo-EM
(2022-07-19)Electron cryomicroscopy (cryo-EM) is a powerful tool in structural biology for determining 3D structures of macromolecular complexes. In the previous decade, many technological advancements have been introduced to improve ... -
Manipulating the Conformational Landscape of Large Macromolecular Complexes
(2022-04-08)The de novo synthesis of fatty acids is one of the most central metabolic pathways in all forms of life. Among all known organisms, fatty acids are highly essential. Besides their role as energy resource, they are also ... -
Metal-induced aggregation of ß-peptides on membrane surfaces
(2022-04-05)According to the liquid mosaic model of Singer and Nicolson, the cell membrane defines the boundaries of a cell to the inside and outside and describes a twodimensional lipid bilayer. Integrated into this lipid bilayer ... -
Structural characterization of human spliceosome activation by cryo-EM
(2022-01-27)Eukaryotic genes are transcribed as precursor mRNA (pre-mRNA), in which coding regions (exons) are interrupted by non-coding regions (introns). Introns are excised and exons are ligated together in a two-step process ... -
Synthesis of Orotidine-5'-monophosphate modifications for the study of the catalytic activity of the enzyme Orotidine-5'-monophosphate decarboxylase
(2022-01-27)In this thesis, the synthesis of OMPD substrate analogs was investigated to further elucidate the mechanism of the OMPD. As inhibiting molecules, 6-thioamido UMP, OMP methyl ester, reduced OMP, and nonpolar substituted ... -
Aufbau einer Silicanin-1-haltigen Modellmembran des Silicafällungsvesikels aus Thalassiosira pseudonana
(2021-12-23)Silica polycondensation occurring in diatom organelles called silica deposition vesicles (SDVs) leads to valve and girdle band formation to complete the biosilica cell wall after cell division. This biosilica shows ... -
Synthesis of β-Peptides for the Organization as Helix Bundles in Lipid Membranes
(2021-12-03)In this work, syntheses and analyses of transmembrane β-peptides were performed. On the one hand, a novel β-peptidic molecular ruler was designed which enables the measurement of near-membrane ion concentrations. On the ... -
Reconstitution of the Integrator complex and its interaction with paused transcription elongation complex of Pol II-DSIF-NELF
(2021-10-19)The Integrator complex (INT) is the latest and largest multi-subunit protein complex to be added to the list of factors involved in RNA Polymerase II (Pol II) transcription. INT consist of 15 subunits with an estimated ... -
Investigation of Peptidic Templates for Bioorthogonal Ligations
(2021-08-16)One of the biggest challenges for biochemical researchers in the postgenomic era remains the elucidation of structure-activity relationships in proteins. While many proteins can be routinely produced by recombinant protein ... -
Design and synthesis of small organic molecules to stabilize ALS-associated SOD1 mutants
(2021-06-25)Amyotrophic Lateral Sclerosis is a globally occurring neurodegenerative disease with serious implications for the patient’s life as well as imposing a heavy economic burden both on the affected individuals and society. Due ... -
Design and synthesis of multifunctional ligands to study and prevent aggregation processes in Amyotrophic Lateral Sclerosis proteins
(2021-02-22)Amyotrophic lateral sclerosis (ALS) is an incurable lethal disease that affects motor neurons and its heterogeneity is reflected in various mutations among the sporadic ALS (sALS) and familial ALS (fALS) disease types. ... -
One-step RESOLFT with a positively switchable RSFP with improved deactivation kinetics
(2020-10-01)Interaction and spatial organization of subcellular structures within living cells are an essential aspect of biological research, but conventional microscopy can elucidate these properties only to a finite extent as it is ... -
Structural and biochemical characterization of c-di-AMP synthesizing enzymes
(2020-06-11)One major concern of today’s life is the increase of antimicrobial resistance and the rising num-ber of multi drug resistant bacterial species. There is an urgent need of identifying new antibi-otic drug targets since ... -
Algorithms for Crystal Structure Determination in Macromolecular Crystallography
(2019-11-25)X-ray crystallography has been the leading technique for structure determination since the discovery of the diffraction of X-rays by crystals by Max von Laue. In this thesis the crystal structure of human ceroid lipofuscinosis ... -
Synthesis of rare nucleobases and artificial nucleotides for investigation of catalytic enzyme activity
(2019-10-25)Enzymes are involved in replication, transcription and translation of DNA, as well as the synthesis of nucleobases. The roles and mechanisms for many of these enzymes stay broadly unknown until today. The herein presented ... -
Specific ubiquitin-dependent protein degradation requires a trimeric CandA complex in Aspergillus nidulans
(2019-05-20)E3 cullin-RING ubiquitin ligase complexes are activated by modification of their cullin scaffold protein with the ubiquitin-like modifier Nedd8. Deneddylases as the COP9 signalosome (CSN) or Den1/A interact and destabilize ...