Functional Analysis of E3 Ubiquitin Ligases in Developing Neurons
von Öyküm Kaplan
Datum der mündl. Prüfung:2019-06-18
Erschienen:2019-08-15
Betreuer:Prof. Dr. Nils Brose
Gutachter:Prof. Dr. Thomas Dresbach
Gutachter:Prof. Dr. Tiago Fleming Outeiro
Dateien
Name:20190809_OykumPhDthesis_SUB.pdf
Size:23.5Mb
Format:PDF
Zusammenfassung
Englisch
Neuronal development starts after the closure of the neural tube when neural stem cells begin to divide asymmetrically at the ventricular zone of the forebrain. Newborn neurons then migrate vertically from the ventricular zone to the cortical plate to reach their destinations and start generating axons and dendrites. After completing neuronal migration, neurons further extend and branch axons and dendrites. In the final step of neuronal development, neurons establish neuronal networks by forming and refining chemical synapses through synaptogenesis and synapse elimination. These four developmental steps are spatially and temporarily regulated by multiple signaling pathways. Ubiquitination, which is a regulatory posttranslational modification, is one of the most crucial processes necessary for such complex synaptic formation. In this study, we demonstrate the roles of Nedd4 family E3 ubiquitin ligases in nerve cells and characterize substrate proteins contributing to the current understanding of molecular and cellular pathologies in neurological diseases.
Keywords: ubiquitination; Nedd4-2; Prr7; learning; memory; hippocampus; LTP