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Characterization of genes differentially regulated after bile acid exposure in Campylobacter jejuni

dc.contributor.advisorGroß, Uwe Prof. Dr.
dc.contributor.authorImada Minatelli, Sabrina Yuri
dc.date.accessioned2019-08-23T09:24:23Z
dc.date.available2019-08-23T09:24:23Z
dc.date.issued2019-08-23
dc.identifier.urihttp://hdl.handle.net/21.11130/00-1735-0000-0003-C19D-2
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-7619
dc.language.isoengde
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc570de
dc.titleCharacterization of genes differentially regulated after bile acid exposure in Campylobacter jejunide
dc.typedoctoralThesisde
dc.contributor.refereeGroß, Uwe Prof. Dr.
dc.date.examination2019-07-03
dc.description.abstractengIn the past decades Campylobacter has raised as the main cause of bacterial gastrointestinal infection worldwide. Campylobacter causes gastrointestinal infection that can vary from asymptomatic, mild to a severe diarrhea. Annually, approximately 246,000 confirmed cases of Campylobacter enteritis are reported in Europe, and 74,000 just in Germany (EFSA 2017). C. jejuni and C. coli are the main species related to human infections (Dasti et al. 2010). Consequently, C. jejuni is recognized as an important public health issue which pronounces the importance of pathogenesis studies of this organism. In this study, novel pathogenicity factors involved in the ability of C. jejuni to adapt to the bile acid rich environment of the human gut are aimed to be identified by the generation of knockout mutants. Candidate genes were mainly chosen from proteomics data generated in our lab that resulted in the identification of differentially expressed proteins after exposure to sublethal concentrations of seven bile acids (Masanta et al. 2018). Ten knockout mutants were generated by the insertion of a kanamycin resistance cassette into the target gene via homologous recombination. Various phenotypic parameters were assessed such as adhesion and invasion into two different host cell types, soft agar motility, autoagglutination, biofilm formation and stress resistance. Surprisingly, from our ten knockout mutants six of them showed a strong coupled phenotype with an unstable motility behavior, an increased adhesion and invasion to Caco2 cell and increased biofilm formation. These phenotypic changes can be interpreted as adaptation processes that prepare the bacteria to better survive stress situations by hiding inside host cells or by biofilm formation. Although the six genes are involved in completely different cellular processes, their deletion seems to mimic at least in parts the effects seen after bile acid exposure, which might be explained by the activation of a common genetic program that prepares the organism to stress situations.de
dc.contributor.coRefereeCommichau, Fabian Moritz Dr.
dc.contributor.thirdRefereeBohne, Wolfgang Dr.
dc.subject.engCampylobacter jejunide
dc.subject.engBiofilmde
dc.subject.engKnockout mutantsde
dc.subject.engBile acidde
dc.identifier.urnurn:nbn:de:gbv:7-21.11130/00-1735-0000-0003-C19D-2-6
dc.affiliation.instituteBiologische Fakultät für Biologie und Psychologiede
dc.subject.gokfullBiologie (PPN619462639)de
dc.identifier.ppn167230766X


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