• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Zentren & Graduiertenschulen
  • GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften
  • Item View
  •   Home
  • Zentren & Graduiertenschulen
  • GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

Mover affects a subpool of primed synaptic vesicles in the mouse calyx of Held

by Ermis Pofantis
Doctoral thesis
Date of Examination:2019-04-11
Date of issue:2019-10-10
Advisor:Prof. Dr. Thomas Dresbach
Referee:Prof. Dr. Nils Brose
Referee:Prof. Dr. Tobias Moser
Referee:Prof. Dr. Erwin Neher
Referee:Ph.d. Camin Dean
Referee:Dr. Luis A. Pardo
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-7598

 

 

Files in this item

Name:Ermis Pofantis - Dissertation.pdf
Size:4.54Mb
Format:PDF
Description:Dissertation
ViewOpen

The following license files are associated with this item:


Abstract

English

Neuronal communication is a complex process; synapses must be formed, neurotransmitter has to be released at precise time points and it has to be “sensed” by the receiving end of a synapse with an equal accuracy. In this highly coordinated ballet of proteins any change may result in disharmony and eventually in pathology. Therefore, any new addition during the course of evolution must be fulfilling a specific purpose. A relatively new protein in the evolutionarily highly-conserved presynaptic apparatus, since it is vertebrate specific, is Mover. It is attached to synaptic vesicles and interacts with Calmodulin and Bassoon, another vertebrate-specific active zone protein. Mover’s expression levels vary throughout the brain, suggesting a modulatory function at the operation of the synapses. Here, I aimed to elucidate Mover’s role in synaptic transmission in the calyx of Held, a central glutamatergic synapse, using a Mover knockout (ko) mice. To this end, I recorded spontaneous and evoked excitatory postsynaptic currents (epscs) from brainstem slices using a whole-cell patch clamp configuration. In the ko evoked epscs were slightly smaller, and took longer to reach the same steady-state levels as the wild-type upon high frequency stimulation. Applying a blind-source separation technique termed non-negative tensor factorization allowed me to distinguish between different subpools of vesicles. This analysis gave rise to a model in which the absence of Mover reduces the release probability of a subpool of vesicles, termed “tight-state” vesicles –referring to the conformation of the snare complex and its associated proteins. Additionally, the size of this pool is significantly increased, indicating a compensatory mechanism. In contrast, the loose-state synaptic vesicles, the functional precursors of the tight-state ones, are unaffected by the absence of Mover. These findings suggest that Mover modulates the initial release probability, by specifically influencing the subpool of these tight-state vesicles.
Keywords: neuroscience; biology; synaptic transmission; synaptic plasticity; calyx of Held; presynapse; Mover; synaptic priming; superpriming; presynaptic proteins; brain; mouse
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Cookie Consents | Data Protection Information | Accessibility
eDiss Office - SUB Göttingen (Central Library)
Platz der Göttinger Sieben 1
Mo - Fr 10:00 – 12:00 h


Tel.: +49 (0)551 39-27809 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
ediss_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]
Göttingen State and University Library | Göttingen University
Medicine Library (Doctoral candidates of medicine only)
Robert-Koch-Str. 40
Mon – Fri 8:00 – 24:00 h
Sat - Sun 8:00 – 22:00 h
Holidays 10:00 – 20:00 h
Tel.: +49 551 39-8395 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
bbmed_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]