Zur Rolle von N-Cadherin in der Proliferation, Migration und Invasion maligner Keimzelltumoren des Hodens

Schallenberg, Simon

dc.contributor.advisor	Bremmer, Felix PD Dr.
dc.contributor.author	Schallenberg, Simon
dc.date.accessioned	2019-11-19T11:08:40Z
dc.date.available	2019-12-19T23:50:02Z
dc.date.issued	2019-11-19
dc.identifier.uri	http://hdl.handle.net/21.11130/00-1735-0000-0005-12AD-4
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7737
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7737
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7737
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Zur Rolle von N-Cadherin in der Proliferation, Migration und Invasion maligner Keimzelltumoren des Hodens	de
dc.type	doctoralThesis	de
dc.title.translated	Role of N-cadherin in proliferation, migration, and invasion of germ cell tumours.	de
dc.contributor.referee	Schön, Margarete Prof. Dr.
dc.date.examination	2019-12-12
dc.description.abstracteng	Germ cell tumors (GCTs) are the most common malignancies in young men. Most patients with GCT can be cured with cisplatin-based combination chemotherapy, even in metastatic disease. In case of therapy resistance, prognosis is usually poor. We investigated the potential of N-cadherin inhibition as a therapeutic strategy. We analyzed the GCT cell lines NCCIT, NTERA-2, TCam-2, and the cisplatin-resistant sublines NCCIT-R and NTERA-2R. Effects of a blocking antibody or siRNA against N-cadherin on proliferation, migration, and invasion were investigated. All investigated GCT cell lines were found to express N-cadherin protein in vitro and in vivo. Downregulation of N-cadherin in vitro leads to a significant inhibition of proliferation, migration, and invasion. N-cadherin-downregulation leads to a significantly higher level of pERK. N-cadherin-inhibition resulted in significantly higher rates of apoptotic cells in caspase-3 staining. Expression of N-cadherin is preserved in cisplatin-resistant GCT cells, pointing to an important physiological role in cell survival. N-cadherin-downregulation results in a significant decrease of proliferation, migration, and invasion and stimulates apoptosis in cisplatin-naive and resistant GCT cell lines. Therefore, targeting N-cadherin may be a promising therapeutic approach, particularly in cisplatin-resistant, therapy refractory and metastatic GCT	de
dc.contributor.coReferee	Meyer, Thomas Prof. Dr.
dc.subject.ger	Keimzelltumorzelllinien	de
dc.subject.ger	Keimzelltumoren	de
dc.subject.ger	Cisplatin-Resistenz	de
dc.subject.ger	Migration	de
dc.subject.ger	Invasion	de
dc.subject.ger	Proliferation	de
dc.subject.ger	N-Cadherin	de
dc.subject.eng	GCT-cell lines	de
dc.subject.eng	germ cell tumours	de
dc.subject.eng	cisplatin resistance	de
dc.subject.eng	migration	de
dc.subject.eng	invasion	de
dc.subject.eng	proliferation	de
dc.subject.eng	N-cadherin	de
dc.identifier.urn	urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-12AD-4-0
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Pathologie / Pathologische Anatomie / Histopathologie / Zytopathologie - Allgemein- und Gesamtdarstellungen (PPN619875674)	de
dc.description.embargoed	2019-12-19
dc.identifier.ppn	1682184471

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