| dc.contributor.advisor | Rizzoli, Silvio O. Prof. Dr. | |
| dc.contributor.author | Jähne, Sebastian | |
| dc.date.accessioned | 2020-04-02T09:31:01Z | |
| dc.date.available | 2020-05-19T22:50:03Z | |
| dc.date.issued | 2020-04-02 | |
| dc.identifier.uri | http://hdl.handle.net/21.11130/00-1735-0000-0005-1374-3 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7934 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 570 | de |
| dc.title | A quantitative analysis of an average cultured neuron | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Rizzoli, Silvio O. Prof. Dr. | |
| dc.date.examination | 2019-05-20 | |
| dc.description.abstracteng | Neurons are the functional unit of the brain. They have been extensively studied, from a molecular perspective up to brain wide levels. Nonetheless, we are still missing a quantitative description of the neuronal components. I present here a comprehensive description of cultured hippocampal neurons and their compartments and organelles. I used a combination of fluorescence microscopy, super-resolution microscopy, and electron microscopy to determine the distributions, volumes, and compartment specific differences of 32 organelle markers. I found that organelles occupy almost 90% of the neuronal cell body, which implicated diffusional constraints. Comparing organelle sizes across axons, dendrites, and cell bodies, I could show that most organelles are significantly different when located in different compartments. Finally, I was able to show that the number of pre- and postsynapses per cultured neuron is tightly correlated despite a strong disparity between the axonal and dendritic volumes. The dataset provided here is the basis for a quantitative molecular nanomap of a cultured hippocampal neuron, which in addition to the organelle composition will contain the molecular composition.
Furthermore, I used correlated optical and isotopic imaging to study the protein turnover at synapses. I found that the presynaptic protein turnover is correlated to synaptic activity at the single synapse level. This is interesting as it shows for the first time a direct coupling of synaptic activity to protein turnover. Chronically inhibiting synaptic activity produced a homeostatic scaling effect with increased protein turnover. | de |
| dc.contributor.coReferee | Brose, Nils Prof. Dr. | |
| dc.subject.eng | hippocampal neuron | de |
| dc.subject.eng | neuron nanomap | de |
| dc.subject.eng | organelle | de |
| dc.subject.eng | synapse turnover | de |
| dc.subject.eng | quantitative neurobiology | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-1374-3-6 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.description.embargoed | 2020-05-19 | |
| dc.identifier.ppn | 1693990946 | |