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A quantitative analysis of an average cultured neuron

dc.contributor.advisorRizzoli, Silvio O. Prof. Dr.
dc.contributor.authorJähne, Sebastian
dc.titleA quantitative analysis of an average cultured neuronde
dc.contributor.refereeRizzoli, Silvio O. Prof. Dr.
dc.description.abstractengNeurons are the functional unit of the brain. They have been extensively studied, from a molecular perspective up to brain wide levels. Nonetheless, we are still missing a quantitative description of the neuronal components. I present here a comprehensive description of cultured hippocampal neurons and their compartments and organelles. I used a combination of fluorescence microscopy, super-resolution microscopy, and electron microscopy to determine the distributions, volumes, and compartment specific differences of 32 organelle markers. I found that organelles occupy almost 90% of the neuronal cell body, which implicated diffusional constraints. Comparing organelle sizes across axons, dendrites, and cell bodies, I could show that most organelles are significantly different when located in different compartments. Finally, I was able to show that the number of pre- and postsynapses per cultured neuron is tightly correlated despite a strong disparity between the axonal and dendritic volumes. The dataset provided here is the basis for a quantitative molecular nanomap of a cultured hippocampal neuron, which in addition to the organelle composition will contain the molecular composition. Furthermore, I used correlated optical and isotopic imaging to study the protein turnover at synapses. I found that the presynaptic protein turnover is correlated to synaptic activity at the single synapse level. This is interesting as it shows for the first time a direct coupling of synaptic activity to protein turnover. Chronically inhibiting synaptic activity produced a homeostatic scaling effect with increased protein
dc.contributor.coRefereeBrose, Nils Prof. Dr.
dc.subject.enghippocampal neuronde
dc.subject.engneuron nanomapde
dc.subject.engsynapse turnoverde
dc.subject.engquantitative neurobiologyde
dc.affiliation.instituteGöttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)de
dc.subject.gokfullBiologie (PPN619462639)de

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