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A quantitative analysis of an average cultured neuron

dc.contributor.advisorRizzoli, Silvio O. Prof. Dr.
dc.contributor.authorJähne, Sebastian
dc.date.accessioned2020-04-02T09:31:01Z
dc.date.available2020-05-19T22:50:03Z
dc.date.issued2020-04-02
dc.identifier.urihttp://hdl.handle.net/21.11130/00-1735-0000-0005-1374-3
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-7934
dc.language.isoengde
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc570de
dc.titleA quantitative analysis of an average cultured neuronde
dc.typedoctoralThesisde
dc.contributor.refereeRizzoli, Silvio O. Prof. Dr.
dc.date.examination2019-05-20
dc.description.abstractengNeurons are the functional unit of the brain. They have been extensively studied, from a molecular perspective up to brain wide levels. Nonetheless, we are still missing a quantitative description of the neuronal components. I present here a comprehensive description of cultured hippocampal neurons and their compartments and organelles. I used a combination of fluorescence microscopy, super-resolution microscopy, and electron microscopy to determine the distributions, volumes, and compartment specific differences of 32 organelle markers. I found that organelles occupy almost 90% of the neuronal cell body, which implicated diffusional constraints. Comparing organelle sizes across axons, dendrites, and cell bodies, I could show that most organelles are significantly different when located in different compartments. Finally, I was able to show that the number of pre- and postsynapses per cultured neuron is tightly correlated despite a strong disparity between the axonal and dendritic volumes. The dataset provided here is the basis for a quantitative molecular nanomap of a cultured hippocampal neuron, which in addition to the organelle composition will contain the molecular composition. Furthermore, I used correlated optical and isotopic imaging to study the protein turnover at synapses. I found that the presynaptic protein turnover is correlated to synaptic activity at the single synapse level. This is interesting as it shows for the first time a direct coupling of synaptic activity to protein turnover. Chronically inhibiting synaptic activity produced a homeostatic scaling effect with increased protein turnover.de
dc.contributor.coRefereeBrose, Nils Prof. Dr.
dc.subject.enghippocampal neuronde
dc.subject.engneuron nanomapde
dc.subject.engorganellede
dc.subject.engsynapse turnoverde
dc.subject.engquantitative neurobiologyde
dc.identifier.urnurn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-1374-3-6
dc.affiliation.instituteGöttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)de
dc.subject.gokfullBiologie (PPN619462639)de
dc.description.embargoed2020-05-19
dc.identifier.ppn1693990946


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