Assoziation psychometrisch erfasster depressiver Symptomatik mit dem Expressionsgrad von NF-kB bei inflammatorischen Erkrankungen des Gastrointestinaltraktes

Association of psychometrically recorded depressive symptoms with the expression level of NF-kB in inflammatory diseases of the gastrointestinal tract

by Lara Marie Vonhören
Doctoral thesis
Date of Examination:2020-06-16
Date of issue:2020-05-29
Advisor:Prof. Dr. Thomas Meyer
Referee:Prof. Dr. Thomas Meyer
Referee:Prof. Dr. Harald Schwörer
Persistent Address: http://dx.doi.org/10.53846/goediss-7980

 

 

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Abstract

English

The transcription factor NF-kB (nuclear factor-kappa B) plays an important role in the regulation of the immune responses, cell proliferation and apoptosis. The present clinical trial tested the hypothesis that there is a positive correlation between NF-kB expression in inflammatory gastrointestinal tissue and psychometrically assessed, self-rated depression. A total of 52 patients were included in this prospective monocenter, observational study in whom biopsies were taken in a gastroscopic (n = 32) or colonoscopic examination (n = 20). In histological sections of the specimens, immunohistochemical stainings were performed to evaluate the level of NF-kB, MPO (myeloperoxidase) and iNOS (inducible nitric oxide synthase) expression. Prior to the endoscopic procedure, self-rated anxiety and depression were assessed using the German version of the Hospital Anxiety and Depression Scale (HADS) and individual quality of life was rated using the SF-36 questionnaire. The results showed that in total 40% (21/52) of the study cohort displayed positive HADS depression scores above the clinically relevant cut-off of ≥8. There was a positive and significant association between HADS depression and the expression level of NF-kB in samples from the upper gastrointestinal tract. Statistically significant relationships between the degree of NF-kB immunoreactivity and the anxiety score or quality of life were not found. However, there was a significant correlation between NF-kB and iNOS expression levels. In summary, the study demonstrated a significant correlation between NF-kB expression in inflammatory gastrointestinal tissue and depression. Evidence of a similar association with comorbid anxiety was not found. Further investigations are needed to confirm these relationships and to identify the involved pathophysiological mechanisms.
Keywords: NF-kB; HADS-D; SF-36; inflammatory gastro-intestinal tissue; depression
 
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