Evaluation der Interaktionen zwischen extrazellulärer Matrix und ausgewählten tumorassoziierten Proteinen mittels Nahinfrarot-Antikörpern

Eckardt, Jan-Niklas

dc.contributor.advisor	Missbach-Güntner, Jeannine Dr.
dc.contributor.author	Eckardt, Jan-Niklas
dc.date.accessioned	2020-10-23T14:06:01Z
dc.date.available	2020-11-10T23:50:02Z
dc.date.issued	2020-10-23
dc.identifier.uri	http://hdl.handle.net/21.11130/00-1735-0000-0005-14BF-E
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-8263
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-8263
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Evaluation der Interaktionen zwischen extrazellulärer Matrix und ausgewählten tumorassoziierten Proteinen mittels Nahinfrarot-Antikörpern	de
dc.type	doctoralThesis	de
dc.title.translated	Evaluation of interactions between the extracellular matrix and selected tumor-associated proteins with near-infrared antibodies	de
dc.contributor.referee	Alves, Frauke Prof. Dr.
dc.date.examination	2020-10-29
dc.description.abstracteng	The interplay between tumor cells and their microenvironment is crucial for carcinogenesis and tumor progression. In my thesis, I examine selected tumor-associated proteins that interact with the extracellular matrix surrounding tumor cells by evaluating their interactions with selected antibodies that are conjugated to near-infrared fluorophors (NIRF) in vitro and in vivo. I demonstrate an identical pattern of biodistribution of NIRF-antibodies independent of tumor model, tumor transplantation mode in vivo, conjugated fluorophore and temporal dynamics. Further, in a two-dimensional in vitro model I demonstrate the upregulation of a selected tumor-associated protein in response to chemotherapy with concomitant cell lysis whereas in a three-dimensional model chemotherapy selected a more aggressive phenotype of tumor cells clearly underlining distinct differences between different dimensional models in cell culture.	de
dc.contributor.coReferee	Wouters-Bunt, Freddy Prof. Dr.
dc.subject.eng	Extracellular Matrix	de
dc.subject.eng	EpCAM	de
dc.subject.eng	Lysyloxidase	de
dc.subject.eng	CD44	de
dc.subject.eng	Tumor Spheroid	de
dc.subject.eng	Near-Infrared Antibody	de
dc.identifier.urn	urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-14BF-E-9
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Innere Medizin - Allgemein- und Gesamtdarstellungen (PPN619875747)	de
dc.description.embargoed	2020-11-10
dc.identifier.ppn	1736532138