| dc.contributor.advisor | Parlitz, Ulrich Prof. Dr. | |
| dc.contributor.author | Kappadan, Vineesh | |
| dc.date.accessioned | 2021-01-06T14:54:40Z | |
| dc.date.available | 2021-01-06T14:54:40Z | |
| dc.date.issued | 2021-01-06 | |
| dc.identifier.uri | http://hdl.handle.net/21.11130/00-1735-0000-0005-1538-5 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-8384 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 530 | de |
| dc.title | Ratiometric fluorescence imaging and marker-free motion tracking of Langendorff perfused beating rabbit hearts | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Parlitz, Ulrich Prof. Dr. | |
| dc.date.examination | 2020-07-14 | |
| dc.subject.gok | Physik (PPN621336750) | de |
| dc.description.abstracteng | Optical mapping is a fluorescence based imaging technique used extensively
in cardiac research to study the electrophysiological properties of isolated
hearts kept at physiological conditions. The major limitation of optical mapping
studies are the distortions of electrophysiological signals due to the
contractile motion of the hearts. To reduce electrophysiological signal distortions
due to the contractile motion artifacts, the mechanical motion has
been suppressed in optical mapping experiments using electromechanical
uncouplers such as Blebbistatin. Recent studies used marker-free numerical
motion tracking and motion stabilization techniques to record electrophysiological
parameters in the absence of electromechanical uncouplers and
showed that contractile motion of the cardiac tissue is no longer a limitation
in optical mapping studies. However, despite these developments, accurate
measurements of quantities such as action potential duration (APD) and
cardiac restitution are still challenging due to the residual motion artifacts
present in the electrophysiological signals even after numerical motion tracking.
A combination of marker-free motion tracking and ratiometric optical
mapping technique is used in this thesis to minimize motion-related artifacts
from contracting hearts. This combined experimental and numerical technique
reduced motion artifacts significantly and hence, the combination is
used to precisely measure APD, cardiac restitution and ventricular fibrillation
frequencies from Langendorff perfused contracting and deforming rabbit
hearts. A systematic comparison of these electrophysiological parameters
in contracting and Blebbistatin-uncoupled conditions showed, on average,
27 ± 5% (N=5 hearts) shortening of APD in contracting hearts as compared
to Blebbistatin-uncoupled hearts. Ventricular fibrillation frequency significantly
increased in contracting hearts (13 ± 3.5 Hz) in comparison with
Blebbistatin-uncoupled hearts (8 ± 1.5 Hz). | de |
| dc.contributor.coReferee | Enderlein, Jörg Prof. Dr. | |
| dc.subject.eng | Optical mapping | de |
| dc.subject.eng | Langendorff perfused hearts | de |
| dc.subject.eng | Motion tracking | de |
| dc.subject.eng | Ratiometry | de |
| dc.subject.eng | Action potential duration | de |
| dc.subject.eng | Ventricular Fibrillation | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-1538-5-8 | |
| dc.affiliation.institute | Fakultät für Physik | de |
| dc.identifier.ppn | 1744143129 | |