Der Einfluss der Induktion von Tumornekrosefaktor α und Transforming-Growth-Factor β auf die epithelial-mesenchymale Transition oraler Plattenepithelkarzinome im CAM-Assay

The impact of the induction of TNF alpha and TGF beta on epithelial-mesenchymal Transition in oral squamous cell carcinoma in the chick chorioallantoic membrane assay

by Gaayathiri Suntharalingam
Doctoral thesis
Date of Examination:2021-02-18
Date of issue:2021-02-09
Advisor:PD Dr. Philipp Kauffmann
Referee:PD Dr. Philipp Kauffmann
Referee:Prof. Dr. Jörg Wilting
Persistent Address: http://dx.doi.org/10.53846/goediss-8435

 

 

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Abstract

English

Introduction: The oral squamous cell carcinoma (OSCC) belongs to one of the highest malignant carcinomas of the upper aerodigestive passages. The aim of this study was to see if the fresh OSCC tumor samples could be used in the CAM assay as a good working in vivo model, which could be integrated into the clinical work flow to evaluate OSCC tumor samples and its growth. Furthermore another main focus of this study was to examine the histological and molecular biology of OSCC with the help of the CAM assay by inducing cytokines TNF α and TGF β on the tumor samples to get a better understanding of the epithelial-to-mesenchymal transition (EMT) mechanism.  Material and methods: Fresh human tumor samples (OSCCs) 1×1 cm in size were cut into 350 μm thick slices and put on the prepared CAM model. After growth of the tumor tissue on the CAM could be detected and induction of TNF α and TGF β were made, the tumor tissue were explanted and immunohistochemically examined. In order to evaluate the effects of the induction the characteristic EMT-makers E-cadherin and vimentin were assessed. Results and Conclusion: This study shows that in fact fresh human OSCC tumor samples with its preserved tumor architecture can be transplanted onto the CAM where they get supplied by the blood vessels of the host, so that the integration of the CAM assay into the clinical workflow is possible. After the induction of TGF β and TNF α mostly no specific reaction regarding the vimentin and E-cadherin expression in the tumor samples could be detected. This means the key markers E-cadherin and vimentin alone are not sufficient to represent the complexity of the EMT. Further molecular and signaling pathway analyzes within the tumor samples are necessary.
Keywords: CAM assay; EMT; oral squamous cell carcinoma; TNF alpha; TGF beta
 
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