| dc.contributor.advisor | Lingor, Paul Prof. Dr. | |
| dc.contributor.author | Schünemann, Jonas Sebastian | |
| dc.date.accessioned | 2021-02-19T11:26:22Z | |
| dc.date.available | 2021-03-25T00:50:03Z | |
| dc.date.issued | 2021-02-19 | |
| dc.identifier.uri | http://hdl.handle.net/21.11130/00-1735-0000-0005-1578-D | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-8429 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 610 | de |
| dc.title | Effects of the microRNA cluster 132/212 in primary dopaminergic neurons | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Lingor, Paul Prof. Dr. | |
| dc.date.examination | 2021-03-18 | |
| dc.description.abstracteng | The microRNA (miRNA) cluster 132/212 is highly involved in the regulation of cell growth and maturation in neurons and other cell types. This work investigated effects of increased levels of the miRNA strands miR-132-3p and miR-212-3p in primary dopaminergic neurons (PMN). Cells transfected with miR-132-3p showed enhanced neurite growth and improved regeneration after mechanical lesion in primary dopaminergic neurons but had an unchanged survival in a cell stress model. Elevated levels of miR-212-3p did not alter the experimental read-outs. In a mass spectrometry approach alteration of the PMN proteome after transfection with the respective protein strands were investigated. ARHGAP32 and MECP were confirmed as targets of miR-132-3p and EPHA5 as a target of miR-212-3p by Western blot. By regulating the expression of the described proteins, the observed effects of elevated miR-132-3p levels on cell growth and regeneration can partly be explained. The underlying mechanism could be an interesting approach for possible therapeutic use in neurodegenerative disease like Parkinson's disease. | de |
| dc.contributor.coReferee | Outeiro, Tiago Fleming Prof. Dr. | |
| dc.contributor.thirdReferee | Meyer, Thomas Prof. Dr. | |
| dc.subject.eng | miR-132/212 cluster | de |
| dc.subject.eng | miR-132 | de |
| dc.subject.eng | miR-212 | de |
| dc.subject.eng | primary dopaminergic neurons | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-1578-D-0 | |
| dc.affiliation.institute | Medizinische Fakultät | de |
| dc.subject.gokfull | Molekularbiologie {Medizin} (PPN619875186) | de |
| dc.subject.gokfull | Neuroanatomie, Neurophysiologie, Neuropathologie (PPN619876255) | de |
| dc.description.embargoed | 2021-03-25 | |
| dc.identifier.ppn | 1748734245 | |