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Design and synthesis of multifunctional ligands to study and prevent aggregation processes in Amyotrophic Lateral Sclerosis proteins

dc.contributor.advisorDiederichsen, Ulf Prof. Dr.
dc.contributor.authorMrđen Debono, Viktoria
dc.publisherNiedersächsische Staats- und Universitätsbibliothek Göttingende
dc.titleDesign and synthesis of multifunctional ligands to study and prevent aggregation processes in Amyotrophic Lateral Sclerosis proteinsde
dc.contributor.refereeDiederichsen, Ulf Prof. Dr.
dc.description.abstractengAmyotrophic lateral sclerosis (ALS) is an incurable lethal disease that affects motor neurons and its heterogeneity is reflected in various mutations among the sporadic ALS (sALS) and familial ALS (fALS) disease types. Superoxide dismutase-1 (SOD1), as a cell guardian, is an essential protein needed for the antioxidative cellular homeostasis but is many times a silent traitor in both types of ALS. The exact mechanisms by which SOD1 mutations contribute to ALS are still not fully understood, but structural investigations have interestingly revealed that the protein is a homodimer. It is suggested that the most severe mutations disrupt the dimer interface, causing dissociation, misfolding, and protein aggregation, which are the main sources of toxicity. Additionally, the aggregation processes trigger many other mechanisms, and correlation between the mutants’ structural damage and aggregation pathways remains a puzzle. In this thesis, in silico docking studies are done to design multifunctional ligands, whose biphenyl core structures mitigate the aggregation of a severe dimer interface mutant. A modular synthetic approach is developed, and different ligands are synthesized to anchor the protein dimer while preventing protein dissociation. Protein-ligand interactions, affinity, and their behaviour are then investigated via microscale thermophoresis, isothermal calorimetry, and aggregation assays. It is shown that synthesized ligands with aromatic substituents have a higher binding affinity toward the protein, and that they could reduce the aggregation of the SOD1 mutant. Additionally, the principle that the ligands can mediate a transfer of a fluorescent dye onto a target amino acid shows their multifunctionality. The design of affinity-based fluorogenic probes is envisaged as an extended approach and should assist to understand the mechanisms leading to SOD1-related
dc.contributor.coRefereeThomas, Franziska Jun.-Prof. Dr
dc.contributor.thirdRefereeTittmann, Kai Prof. Dr.
dc.contributor.thirdRefereeAckermann, Lutz Prof. Dr.
dc.contributor.thirdRefereeOpazo Dávila, Luis Felipe Dr.
dc.contributor.thirdRefereeFrauendorf, Holm Dr.
dc.subject.engAmyotrophic lateral sclerosisde
dc.subject.engSuperoxide dismutase-1de
dc.subject.engprotein aggregationde
dc.subject.engaggregation assaysde
dc.affiliation.instituteFakultät für Chemiede
dc.subject.gokfullChemie  (PPN62138352X)de

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