Identifizierung von neuen Komponenten oder Cargoes der COPI Vesikelhüll Maschinerie

Identification of new components or cargoes of COPI-coated vesicles

by Julia Franz née Grundmeier
Doctoral thesis
Date of Examination:2021-04-12
Date of issue:2021-03-26
Advisor:Prof. Dr. Blanche Schwappach-pignataro
Referee:Prof. Dr. Michael Thumm
Referee:Prof. Dr. Rainer Mausberg
Persistent Address: http://dx.doi.org/10.53846/goediss-8497

 

 

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Abstract

English

Within the cell transport vesicles play an essential role in ensuring the functionality of the cell. The heptamere COPI is responsible for transport from the Golgi to the endoplasmic reticulum and also for transport between the cisternae within the Golgi.Studies have shown the dispensability of the μ-homology domain, which is part of delta-COP. Given the thesis that the μ-homology domain is involved in the triad connection, this is a remarkable finding. In order to better understand the fundamental role and current research findings of the μ homology of the delta-COP subunit in eukaryotic systems, a high-content GFP-based localization screening was carried out. The localization of a subset of the yeast proteom enriched with cargoes and machinery components of the vesicular transport was compared with the help of automated microscopy and manual image analysis in a strain with delta-COP with and without μ-homology domain
Keywords: delta-COPI; COPI; Coatomer; Cargoes; Vesicles
 
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