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In-vivo-Biofilmakkumulation auf implantatprothetischen Werkstoffen

In vivo biofilm accumulation on implant abutment materials

by Andreas Wiessner
Doctoral thesis
Date of Examination:2021-07-14
Date of issue:2021-07-12
Advisor:Prof. Dr. Ralf Bürgers
Referee:Prof. Dr. Tina Rödig
Referee:Prof. Dr. Margarete Schön
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-8728

 

 

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Abstract

English

AIM. The aim of this in vivo study was to investigate the biofilm formation on the surface of four different implant abutment materials (titanium, zirconia, PEEK, BioHPP). MATERIAL/METHODS. Split specimens were developed and mounted in individual removable acrylic upper jaw splints (4 specimens in every splint) and worn by 20 healthy volunteers for 24 h. All specimens were polished and half of them were then artificially aged. The surface roughness was determined using widefield confocal microscopy. Biofilm accumulation was analyzed by using fluorescence staining and microscopy. RESULTS. The surface roughness showed no significant difference between the materials. Zirconia conducted the lowest biofilm mass followed by titanium. BioHPP showed the highest biofilm mass. There were significant differences in microbial colonization between the individual materials except for BioHPP and regular PEEK. Specimes located in the posterior region of the splints showed a significant higher biofilm aggregation than those positioned in the anterior region. CONCLUSION.The significant differences in initial microbial adhesion between all materials apart from PEEK and BioHPP within 24 hours indicated that the careful choice of the abutment material can influence the tendency of biofilm accumulation and therefore reduce the risk of developing a periimplantitis and consequently a possible implant loss.
Keywords: implant abutments; biofilm accumulation; bacterial adhesion; surface roughness; fluorescence microscopy
Schlagwörter: Implantat Abutment; Biofilm; Plaqueakkumulation; Rauheit; Fluoreszenzmikroskopie
 

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