Herstellung von katalytischen monoklonalen Antikörpern

Djalali Bazzaz, Farrokh

dc.contributor.advisor	Peters, Johann Hinrich Prof. Dr.	de
dc.contributor.author	Djalali Bazzaz, Farrokh	de
dc.date.accessioned	2012-04-16T15:00:59Z	de
dc.date.available	2013-01-30T23:50:40Z	de
dc.date.issued	2001-01-15	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0006-AD3B-8	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-675
dc.format.mimetype	ContentType:application/pdf Size:849	de
dc.language.iso	ger	de
dc.rights.uri	http://webdoc.sub.gwdg.de/diss/copyrdiss.htm	de
dc.title	Herstellung von katalytischen monoklonalen Antikörpern	de
dc.type	doctoralThesis	de
dc.title.translated	Generation of catalytic monoclonal antibodies	de
dc.contributor.referee	Grossbach, Ulrich Prof. Dr.	de
dc.date.examination	2000-10-31	de
dc.subject.dnb	570 Biowissenschaften, Biologie	de
dc.description.abstracteng	Utilizing the similar binding characteristics of substrates to enzymes and antigens to antibodies, respectively, antibodies with catalytic activities have been created since 1986. We have succeeded in developing the first monoclonal antibody that catalyzes a cyclic reaction to form vitamin E in a stereoselective manner. So far, vitamin E can chemically be synthesized in a mixture of eight stereoisomers which have only a limited applicability for human use. Haptens and substrates have been synthesized with predefined entantiomeric key structures representing the chroman ring system of vitamin E. Mice strains with a higher property of forming catalytic antibodies were immunized with KLH conjugated hapten. The monoclonal antibodies developed were prescreened for affinity and thereafter for their catalytic activity. For this purpose a novel cat-ELISA was designed by indirectly immobilizing the antibody to the solid phase. This enabled us to recover substrate as well as product in a soluble form and to further chemically characterize them. The cat-reaction was run in pure methanol which may be the first example of a non-aqueous antibody reaction. The cat-mAb Doro-1 catalyses the syntheses of alpha-tocopherol with 5% product and an ee-value up to 42% measured by TLC and HPLC, (respectively).Moreover we show that a specific monoclonal antibody against vitamin E (mAb Ing-1, an IgG2a, with kappa light chain and a dissociation constant of KD= 6.72 +/- 2.10 x 10-9 M) have been generated and used for low level detection of vitamin E.	de
dc.contributor.coReferee	Schürmann, Friedrich-Wilhelm Prof. Dr.	de
dc.subject.topic	Mathematics and Computer Science	de
dc.subject.eng	catalytic antibody	de
dc.subject.eng	cat-ELISA	de
dc.subject.eng	Vitamin E	de
dc.subject.eng	Hapten	de
dc.subject.bk	58.30 und 44.45	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-987-3	de
dc.identifier.purl	webdoc-987	de
dc.affiliation.institute	Biologische Fakultät inkl. Psychologie	de
dc.subject.gokfull	WA und MED 341	de
dc.identifier.ppn	336084137

Dateien

Name:diss.pdf

Größe:848.5Kb

Format:PDF

Beschreibung:Dissertation

Öffnen

Name:: diss.pdf
Größe:: 848.5Kb
Format:: PDF
Beschreibung:: Dissertation

Öffnen

Das Dokument erscheint in:

Fakultät für Biologie und Psychologie (inkl. GAUSS) [1621]

Zur Kurzanzeige