Auswirkung von Bisphosphonaten auf die Expression von Osteoprotegerin (OPG) und Receptor activator of nuclear factor êB ligand (RANKL) in Osteoblastenkulturen aus Unterkiefer und Becken - Eine Pilotstudie am Hausschwein

Sievers, Niklas

dc.contributor.advisor	Gruber, Rudolf PD Dr. Dr.	de
dc.contributor.author	Sievers, Niklas	de
dc.date.accessioned	2013-01-14T15:32:46Z	de
dc.date.available	2013-01-30T23:50:53Z	de
dc.date.issued	2012-10-26	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F01B-F	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-1628
dc.description.abstract	In der vorliegenden Studie wird die Auswirkung von Bisphosphonaten in verschiedenen Dosierungen auf Präosteoblasten-Zellkulturen aus Unterkiefer und Becken von 5 Hausschweinen evaluiert. Dabei werden dosisabhängig die Proliferationsraten der Zellkulturen sowie die Expression von OPG (Osteoprotegerin) und RANK-L (Receptor activator of nuklear factor kappa B ligand) auf mRNA Ebene durch eine RT-PCR untersucht. Zunächst wurden osteoblastäre Zellen aus Knochenproben des Unterkiefers und des Beckens von Hausschweinen (n=5) im Monolayer kultiviert und charakterisiert. Anschließend erfolgte die Kultivierung der Zellen in Kulturmedien mit einem Zusatz von Bisphosphonaten in den Konzentrationen 10	de
dc.format.mimetype	application/pdf	de
dc.language.iso	ger	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Auswirkung von Bisphosphonaten auf die Expression von Osteoprotegerin (OPG) und Receptor activator of nuclear factor êB ligand (RANKL) in Osteoblastenkulturen aus Unterkiefer und Becken - Eine Pilotstudie am Hausschwein	de
dc.type	doctoralThesis	de
dc.title.translated	Effect of bisphosphonates on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) in osteoblast cultures of the mandible and pelvis - A pilot study on the domestic pig	de
dc.contributor.referee	Gruber, Rudolf PD Dr. Dr.	de
dc.date.examination	2012-11-14	de
dc.subject.dnb	610 Medizin, Gesundheit	de
dc.subject.gok	MED 562	de
dc.description.abstracteng	The present study evaluates the effects of bisphosphonates at different doses on preosteoblast cell cultures from the mandible and pelvis of 5 domestic pigs. The proliferation rates of cell cultures and the expression of OPG (Osteoprotegerin) and RANKL (receptor activator of nuclear factor kappa B ligand) were examined at the mRNA level by RT-PCR in a dose dependent manner. At first the osteoblastic cells from bone samples of the lower jaw and pelvis of domestic pigs (n = 5) were cultured and characterized in a monolayer. This was followed by culturing of the cells in culture media with an addition of bisphosphonates in the concentrations of 10-4M, 10-6M, 10-8M and a control without the addition of bisphosphonates. At the times of 0, 1, 4, 7 and 10 days respectively the cell counts were determined and the expression of OPG and RANKL was evaluated at the mRNA level by RT-PCR analysis. The assessment of proliferation showed an increase in the cell numbers after 10 days at concentration levels of 10-6M, 10-8M and the respective control by factors ranging between 4.80 and 6.16 for cells of the pelvis and by factors 5.41 to 7.27 for cells of the mandible. The cells of 10-4M concentration showed a significant reduction in the number of cells to 18.30% of the starting cell number for the preosteoblasts of the pelvis and to 20.15% for preosteoblasts of the mandible. Demonstrated in the assessment of the expression of OPG, a significant dependence of the culture period was showed (p <0.01) with a decrease of expression after the first day and an increase in expression until tenth day of cultivation. It was found that the expression curves for the cells of the lower jaw run at a lower level than the cells of the pelvis (p <0.01). Furthermore, a significant effect of the bisphosphonate concentration on the expression of OPG was demonstrated (p <0.01), but not on the expression of RANKL (p = 0.81). The expression analysis of RANKL resulted in no significant differences between cells from pelvis or lower jaw (p = 0.95), while a significant influence of the culture period could be demonstrated (p <0.01). This resulted in an increase of the expression levels of RANKL with log2 factor of 0.2 after 10 days culture period. The experiments show a divergent behavior of osteoblastic cells from pelvis and mandible for the expression of OPG, whereas the expression of RANKL displays no significant differences. Possibly, these divergent expressions of OPG may give an indication in the pathogenesis of bisphosphonate-associated ONJ.	de
dc.contributor.coReferee	Raddatz, Dirk Prof. Dr.	de
dc.subject.topic	Medicine	de
dc.subject.ger	Bisphosphonate	de
dc.subject.ger	BP-ONJ	de
dc.subject.ger	Kiefernekrose	de
dc.subject.ger	Becken	de
dc.subject.ger	Unterkiefer	de
dc.subject.ger	RANKL	de
dc.subject.ger	OPG	de
dc.subject.ger	Hausschwein	de
dc.subject.eng	Bisphosphonates	de
dc.subject.eng	BP-ONJ	de
dc.subject.eng	ONJ	de
dc.subject.eng	pelvis	de
dc.subject.eng	mandible	de
dc.subject.eng	RANKL	de
dc.subject.eng	OPG	de
dc.subject.eng	pig	de
dc.subject.bk	44.96	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3752-6	de
dc.identifier.purl	webdoc-3752	de
dc.affiliation.institute	Medizinische Fakultät	de
dc.identifier.ppn	734331991	de

Dateien

Name:sievers.pdf

Größe:20.97Mb

Format:PDF

Öffnen

Name:: sievers.pdf
Größe:: 20.97Mb
Format:: PDF

Öffnen

Das Dokument erscheint in:

Human- und Zahnmedizin [2873]

Zur Kurzanzeige